Isolation, separation, and detection of enkephalins: A review of methods for high performance liquid chromatog-raphy and capillary electrophoresis

W. Jeffrey Hurst, Ian Zagon

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

High Performance Liquid Chromatography(HPLC) and Capillary Electrophoresis (CE) are valuable analytical techniques for the determination of neuropeptides in biological fluids. A crucial aspect of HPLC and CE analyses is the preparation of samples. This review outlines sample preparation protocols for HPLC and CE methods that have been used for this determination, with a focus on enkephalins. Sample preparation protocols have ranged from simple techniques of extraction and centrifugation to solid phase extraction (SPE) and ultrafiltration. Additionally, this review also discusses the final determinant steps of HPLC and CE using various modes of detection. Detection modes for the HPLC methods included ultraviolet (UV), fluorometry, electrochemical and mass spectrometry (MS). Modes of detection when using CE for this determination have been more limited, focusing on UV and MS detection modes.

Original languageEnglish (US)
Pages (from-to)2943-2967
Number of pages25
JournalJournal of Liquid Chromatography
Volume18
Issue number15
DOIs
StatePublished - Sep 1 1995

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Capillary electrophoresis
Enkephalins
Capillary Electrophoresis
High performance liquid chromatography
High Pressure Liquid Chromatography
Liquids
Mass spectrometry
Mass Spectrometry
Fluorometry
Centrifugation
Solid Phase Extraction
Ultrafiltration
Neuropeptides
Fluids

All Science Journal Classification (ASJC) codes

  • Molecular Medicine

Cite this

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