Juvenile hormone regulates Aedes aegypti Krüppel homolog 1 through a conserved E box motif

Yingjun Cui, Yipeng Sui, Jingjing Xu, Fang Zhu, Subba Reddy Palli

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Juvenile hormone (JH) plays important roles in regulation of many physiological processes including development, reproduction and metabolism in insects. However, the molecular mechanisms of JH signaling pathway are not completely understood. To elucidate the molecular mechanisms of JH regulation of Krüppel homolog 1 gene (Kr-h1) in Aedes aegypti, we employed JH-sensitive Aag-2cells developed from the embryos of this insect. In Aag-2cells, AaKr-h1 gene is induced by nanomolar concentration of JH III, its expression peaked at 1.5h after treatment with JH III. RNAi studies showed that JH induction of this gene requires the presence of Ae. aegypti methoprene-tolerant (AaMet). A conserved 13 nucleotide JH response element (JHRE, TGCCTC. CACGTGC) containing canonical E box motif (underlined) identified in the promoter of AaKr-h1 is required for JH induction of this gene. Critical nucleotides in the JHRE required for JH action were identified by employing mutagenesis and reporter assays. Reporter assays also showed that basic helix loop helix (bHLH) domain of AaMet is required for JH induction of AaKr-h1. 5' rapid amplification of cDNA ends method identified two isoforms of AaKr-h1, AaKr-h1α and AaKr-h1β, the expression of both isoforms is induced by JH III, but AaKr-h1α is the predominant isoform in both Aag-2cells and Ae. aegypti larvae.

Original languageEnglish (US)
Pages (from-to)23-32
Number of pages10
JournalInsect Biochemistry and Molecular Biology
Volume52
Issue number1
DOIs
StatePublished - Sep 2014

Fingerprint

E-Box Elements
Juvenile Hormones
Aedes
juvenile hormones
Aedes aegypti
Methoprene
Genes
Protein Isoforms
methoprene
Insects
gene induction
Assays
Helix-Loop-Helix Motifs
Nucleotides
Physiological Phenomena
Mutagenesis
nucleotides
Response Elements
RNA Interference
Metabolism

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Insect Science

Cite this

Cui, Yingjun ; Sui, Yipeng ; Xu, Jingjing ; Zhu, Fang ; Palli, Subba Reddy. / Juvenile hormone regulates Aedes aegypti Krüppel homolog 1 through a conserved E box motif. In: Insect Biochemistry and Molecular Biology. 2014 ; Vol. 52, No. 1. pp. 23-32.
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abstract = "Juvenile hormone (JH) plays important roles in regulation of many physiological processes including development, reproduction and metabolism in insects. However, the molecular mechanisms of JH signaling pathway are not completely understood. To elucidate the molecular mechanisms of JH regulation of Kr{\"u}ppel homolog 1 gene (Kr-h1) in Aedes aegypti, we employed JH-sensitive Aag-2cells developed from the embryos of this insect. In Aag-2cells, AaKr-h1 gene is induced by nanomolar concentration of JH III, its expression peaked at 1.5h after treatment with JH III. RNAi studies showed that JH induction of this gene requires the presence of Ae. aegypti methoprene-tolerant (AaMet). A conserved 13 nucleotide JH response element (JHRE, TGCCTC. CACGTGC) containing canonical E box motif (underlined) identified in the promoter of AaKr-h1 is required for JH induction of this gene. Critical nucleotides in the JHRE required for JH action were identified by employing mutagenesis and reporter assays. Reporter assays also showed that basic helix loop helix (bHLH) domain of AaMet is required for JH induction of AaKr-h1. 5' rapid amplification of cDNA ends method identified two isoforms of AaKr-h1, AaKr-h1α and AaKr-h1β, the expression of both isoforms is induced by JH III, but AaKr-h1α is the predominant isoform in both Aag-2cells and Ae. aegypti larvae.",
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Juvenile hormone regulates Aedes aegypti Krüppel homolog 1 through a conserved E box motif. / Cui, Yingjun; Sui, Yipeng; Xu, Jingjing; Zhu, Fang; Palli, Subba Reddy.

In: Insect Biochemistry and Molecular Biology, Vol. 52, No. 1, 09.2014, p. 23-32.

Research output: Contribution to journalArticle

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AU - Sui, Yipeng

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