Kinesin-2 and Apc function at dendrite branch points to resolve microtubule collisions

Alexis T. Weiner, Michael C. Lanz, Daniel J. Goetschius, William O. Hancock, Melissa M. Rolls

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

In Drosophila neurons, kinesin-2, EB1 and Apc are required to maintain minus-end-out dendrite microtubule polarity, and we previously proposed they steer microtubules at branch points. Motor-mediated steering of microtubule plus ends could be accomplished in two ways: 1) by linking a growing microtubule tip to the side of an adjacent microtubule as it navigates the branch point (bundling), or 2) by directing a growing microtubule after a collision with a stable microtubule (collision resolution). Using live imaging to distinguish between these two mechanisms, we found that reduction of kinesin-2 did not alter the number of microtubules that grew along the edge of the branch points where stable microtubules are found. However, reduction of kinesin-2 or Apc did affect the number of microtubules that slowed down or depolymerized as they encountered the side of the branch opposite to the entry point. These results are consistent with kinesin-2 functioning with Apc to resolve collisions. However, they do not pinpoint stable microtubules as the collision partner as stable microtubules are typically very close to the membrane. To determine whether growing microtubules were steered along stable ones after a collision, we analyzed the behavior of growing microtubules at dendrite crossroads where stable microtubules run through the middle of the branch point. In control neurons, microtubules turned in the middle of the crossroads. However, when kinesin-2 was reduced some microtubules grew straight through the branch point and failed to turn. We propose that kinesin-2 functions to steer growing microtubules along stable ones following collisions.

Original languageEnglish (US)
Pages (from-to)35-44
Number of pages10
JournalCytoskeleton
Volume73
Issue number1
DOIs
StatePublished - Jan 1 2016

Fingerprint

Kinesin
Dendrites
Microtubules
Neurons

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Cell Biology

Cite this

Weiner, Alexis T. ; Lanz, Michael C. ; Goetschius, Daniel J. ; Hancock, William O. ; Rolls, Melissa M. / Kinesin-2 and Apc function at dendrite branch points to resolve microtubule collisions. In: Cytoskeleton. 2016 ; Vol. 73, No. 1. pp. 35-44.
@article{c139fdaa77d64603a211e1a1dd3037b1,
title = "Kinesin-2 and Apc function at dendrite branch points to resolve microtubule collisions",
abstract = "In Drosophila neurons, kinesin-2, EB1 and Apc are required to maintain minus-end-out dendrite microtubule polarity, and we previously proposed they steer microtubules at branch points. Motor-mediated steering of microtubule plus ends could be accomplished in two ways: 1) by linking a growing microtubule tip to the side of an adjacent microtubule as it navigates the branch point (bundling), or 2) by directing a growing microtubule after a collision with a stable microtubule (collision resolution). Using live imaging to distinguish between these two mechanisms, we found that reduction of kinesin-2 did not alter the number of microtubules that grew along the edge of the branch points where stable microtubules are found. However, reduction of kinesin-2 or Apc did affect the number of microtubules that slowed down or depolymerized as they encountered the side of the branch opposite to the entry point. These results are consistent with kinesin-2 functioning with Apc to resolve collisions. However, they do not pinpoint stable microtubules as the collision partner as stable microtubules are typically very close to the membrane. To determine whether growing microtubules were steered along stable ones after a collision, we analyzed the behavior of growing microtubules at dendrite crossroads where stable microtubules run through the middle of the branch point. In control neurons, microtubules turned in the middle of the crossroads. However, when kinesin-2 was reduced some microtubules grew straight through the branch point and failed to turn. We propose that kinesin-2 functions to steer growing microtubules along stable ones following collisions.",
author = "Weiner, {Alexis T.} and Lanz, {Michael C.} and Goetschius, {Daniel J.} and Hancock, {William O.} and Rolls, {Melissa M.}",
year = "2016",
month = "1",
day = "1",
doi = "10.1002/cm.21270",
language = "English (US)",
volume = "73",
pages = "35--44",
journal = "Cytoskeleton",
issn = "1949-3584",
publisher = "Wiley-Liss Inc.",
number = "1",

}

Kinesin-2 and Apc function at dendrite branch points to resolve microtubule collisions. / Weiner, Alexis T.; Lanz, Michael C.; Goetschius, Daniel J.; Hancock, William O.; Rolls, Melissa M.

In: Cytoskeleton, Vol. 73, No. 1, 01.01.2016, p. 35-44.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Kinesin-2 and Apc function at dendrite branch points to resolve microtubule collisions

AU - Weiner, Alexis T.

AU - Lanz, Michael C.

AU - Goetschius, Daniel J.

AU - Hancock, William O.

AU - Rolls, Melissa M.

PY - 2016/1/1

Y1 - 2016/1/1

N2 - In Drosophila neurons, kinesin-2, EB1 and Apc are required to maintain minus-end-out dendrite microtubule polarity, and we previously proposed they steer microtubules at branch points. Motor-mediated steering of microtubule plus ends could be accomplished in two ways: 1) by linking a growing microtubule tip to the side of an adjacent microtubule as it navigates the branch point (bundling), or 2) by directing a growing microtubule after a collision with a stable microtubule (collision resolution). Using live imaging to distinguish between these two mechanisms, we found that reduction of kinesin-2 did not alter the number of microtubules that grew along the edge of the branch points where stable microtubules are found. However, reduction of kinesin-2 or Apc did affect the number of microtubules that slowed down or depolymerized as they encountered the side of the branch opposite to the entry point. These results are consistent with kinesin-2 functioning with Apc to resolve collisions. However, they do not pinpoint stable microtubules as the collision partner as stable microtubules are typically very close to the membrane. To determine whether growing microtubules were steered along stable ones after a collision, we analyzed the behavior of growing microtubules at dendrite crossroads where stable microtubules run through the middle of the branch point. In control neurons, microtubules turned in the middle of the crossroads. However, when kinesin-2 was reduced some microtubules grew straight through the branch point and failed to turn. We propose that kinesin-2 functions to steer growing microtubules along stable ones following collisions.

AB - In Drosophila neurons, kinesin-2, EB1 and Apc are required to maintain minus-end-out dendrite microtubule polarity, and we previously proposed they steer microtubules at branch points. Motor-mediated steering of microtubule plus ends could be accomplished in two ways: 1) by linking a growing microtubule tip to the side of an adjacent microtubule as it navigates the branch point (bundling), or 2) by directing a growing microtubule after a collision with a stable microtubule (collision resolution). Using live imaging to distinguish between these two mechanisms, we found that reduction of kinesin-2 did not alter the number of microtubules that grew along the edge of the branch points where stable microtubules are found. However, reduction of kinesin-2 or Apc did affect the number of microtubules that slowed down or depolymerized as they encountered the side of the branch opposite to the entry point. These results are consistent with kinesin-2 functioning with Apc to resolve collisions. However, they do not pinpoint stable microtubules as the collision partner as stable microtubules are typically very close to the membrane. To determine whether growing microtubules were steered along stable ones after a collision, we analyzed the behavior of growing microtubules at dendrite crossroads where stable microtubules run through the middle of the branch point. In control neurons, microtubules turned in the middle of the crossroads. However, when kinesin-2 was reduced some microtubules grew straight through the branch point and failed to turn. We propose that kinesin-2 functions to steer growing microtubules along stable ones following collisions.

UR - http://www.scopus.com/inward/record.url?scp=84956679366&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84956679366&partnerID=8YFLogxK

U2 - 10.1002/cm.21270

DO - 10.1002/cm.21270

M3 - Article

C2 - 26785384

AN - SCOPUS:84956679366

VL - 73

SP - 35

EP - 44

JO - Cytoskeleton

JF - Cytoskeleton

SN - 1949-3584

IS - 1

ER -