Kinetic flux profiling elucidates two independent acetyl-coa biosynthetic pathways in plasmodium falciparum

Simon A. Cobbold, Ashley M. Vaughan, Ian A. Lewis, Heather J. Painter, Nelly Camargo, David H. Perlman, Matthew Fishbaugher, Julie Healer, Alan F. Cowman, Stefan H.I. Kappe, Manuel Llinás

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43 Scopus citations

Abstract

Background: The acetyl-CoA biosynthetic pathways of the malaria parasite are unclear. Results: 13C-Labeling experiments in parasites lacking a functional pyruvate dehydrogenase (PDH) complex show that thePDH does not contribute significantly to the acetyl-CoA pool. Conclusion: The majority of acetyl-CoA biosynthesis in the parasite derives from a PDH-like enzyme and acetyl-CoA synthetase. Significance: The two routes for acetyl-CoA synthesis appear to have separate functions.

Original languageEnglish (US)
Pages (from-to)36338-36350
Number of pages13
JournalJournal of Biological Chemistry
Volume288
Issue number51
DOIs
StatePublished - Dec 20 2013

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Cobbold, S. A., Vaughan, A. M., Lewis, I. A., Painter, H. J., Camargo, N., Perlman, D. H., Fishbaugher, M., Healer, J., Cowman, A. F., Kappe, S. H. I., & Llinás, M. (2013). Kinetic flux profiling elucidates two independent acetyl-coa biosynthetic pathways in plasmodium falciparum. Journal of Biological Chemistry, 288(51), 36338-36350. https://doi.org/10.1074/jbc.M113.503557