Kinetic study on anaerobic oxidation of methane coupled to denitrification

Hou Yu, Hiroyuki Kashima, John M. Regan, Abid Hussain, Elsayed Elbeshbishy, Hyung Sool Lee

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Monod kinetic parameters provide information required for kinetic analysis of anaerobic oxidation of methane coupled to denitrification (AOM-D). This information is critical for engineering AOM-D processes in wastewater treatment facilities. We first experimentally determined Monod kinetic parameters for an AOM-D enriched culture and obtained the following values: maximum specific growth rate (μmax) 0.121/d, maximum substrate-utilization rate (qmax) 28.8 mmol CH4/g cells-d, half maximum-rate substrate concentration (Ks) 83 μΜ CH4, growth yield (Y) 4.76 g cells/mol CH4, decay coefficient (b) 0.031/d, and threshold substrate concentration (Smin) 28.8 μM CH4. Clone library analysis of 16S rRNA and mcrA gene fragments suggested that AOM-D reactions might have occurred via the syntrophic interaction between denitrifying bacteria (e.g., Ignavibacterium, Acidovorax, and Pseudomonas spp.) and hydrogenotrophic methanogens (Methanobacterium spp.), supporting reverse methanogenesis-dependent AOM-D in our culture. High μmax and qmax, and low Ks for the AOM-D enrichment imply that AOM-D could play a significant role in mitigating atmospheric methane efflux. In addition, these high kinetic features suggest that engineered AOM-D systems may provide a sustainable alternative to nitrogen removal in wastewater treatment.

Original languageEnglish (US)
Pages (from-to)47-55
Number of pages9
JournalEnzyme and Microbial Technology
Volume104
DOIs
StatePublished - Sep 2017

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

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