Kinetics of GLUT1 and GLUT4 glucose transporters expressed in Xenopus oocytes

H. Nishimura, F. V. Pallardo, G. A. Seidner, S. Vannucci, Ian Simpson, M. J. Birnbaum

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Abstract

The predominant mechanism by which insulin activates glucose transport in muscle and adipose tissue is by affecting the redistribution of the facilitated hexose carriers, GLUT1 and GLUT4, from an intracellular site to the plasma membrane. A quantitative analysis of this process has been hampered by the lack of reliable determinations for kinetic constants catalyzed by each of these isoforms. In order to obtain such information, each transporter was expressed in Xenopus oocytes by the injection of mRNA encoding rat GLUT1 or GLUT4. Equilibrium exchange 3-O-methylglucose uptake was measured and the data fitted to a two-compartment model, yielding K(m) = 26.2 mM and V(max) = 3.5 nmol/min/cell for GLUT1 and K(m) = 4.3 mM and V(max) = 0.7 nmol/min/cell for GLUT4. Measurement of the abundance of cell surface transporters was accomplished by two independent protocols: photolabeling with the impermeant hexose analog 2-N-4-(1-azi-2,2,2-trifluoroethyl)benzoyl- 1,3-bis(D-mannos-4-yloxy)-2-propylamine and subcellular fractionation of oocytes. Data obtained by either technique revealed that the ratio of plasma membrane GLUT1 to GLUT4 was about 4; this paralleled the relative maximal velocities for hexose transport, indicating that the turnover numbers for the two isoforms were the same. Moreover, measurement of the concentration of exofacially disposed transporters with 2-N-4-(1-azi-2,2,2- trifluoroethyl)benzoyl-1,3-bis(D-mannos-4-yloxy)-2-propylamine allowed calculation of the turnover number to be about 20,000 min-1. These data indicate that, at low substrate concentrations, the catalytic efficiency of GLUT4 is significantly greater than GLUT1. Extrapolation to mammalian systems suggests that GLUT4 is responsible for virtually all of the hexose uptake in insulin-responsive targets, particularly in the presence of hormone.

Original languageEnglish (US)
Pages (from-to)8514-8520
Number of pages7
JournalJournal of Biological Chemistry
Volume268
Issue number12
StatePublished - Jan 1 1993

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Hexoses
Facilitative Glucose Transport Proteins
Xenopus
Oocytes
Kinetics
Cell membranes
Protein Isoforms
Cell Membrane
3-O-Methylglucose
Insulin
Fractionation
Extrapolation
Muscle
Adipose Tissue
Rats
Hormones
Tissue
Glucose
Muscles
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Nishimura, H., Pallardo, F. V., Seidner, G. A., Vannucci, S., Simpson, I., & Birnbaum, M. J. (1993). Kinetics of GLUT1 and GLUT4 glucose transporters expressed in Xenopus oocytes. Journal of Biological Chemistry, 268(12), 8514-8520.
Nishimura, H. ; Pallardo, F. V. ; Seidner, G. A. ; Vannucci, S. ; Simpson, Ian ; Birnbaum, M. J. / Kinetics of GLUT1 and GLUT4 glucose transporters expressed in Xenopus oocytes. In: Journal of Biological Chemistry. 1993 ; Vol. 268, No. 12. pp. 8514-8520.
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Nishimura, H, Pallardo, FV, Seidner, GA, Vannucci, S, Simpson, I & Birnbaum, MJ 1993, 'Kinetics of GLUT1 and GLUT4 glucose transporters expressed in Xenopus oocytes', Journal of Biological Chemistry, vol. 268, no. 12, pp. 8514-8520.

Kinetics of GLUT1 and GLUT4 glucose transporters expressed in Xenopus oocytes. / Nishimura, H.; Pallardo, F. V.; Seidner, G. A.; Vannucci, S.; Simpson, Ian; Birnbaum, M. J.

In: Journal of Biological Chemistry, Vol. 268, No. 12, 01.01.1993, p. 8514-8520.

Research output: Contribution to journalArticle

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T1 - Kinetics of GLUT1 and GLUT4 glucose transporters expressed in Xenopus oocytes

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AU - Pallardo, F. V.

AU - Seidner, G. A.

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AB - The predominant mechanism by which insulin activates glucose transport in muscle and adipose tissue is by affecting the redistribution of the facilitated hexose carriers, GLUT1 and GLUT4, from an intracellular site to the plasma membrane. A quantitative analysis of this process has been hampered by the lack of reliable determinations for kinetic constants catalyzed by each of these isoforms. In order to obtain such information, each transporter was expressed in Xenopus oocytes by the injection of mRNA encoding rat GLUT1 or GLUT4. Equilibrium exchange 3-O-methylglucose uptake was measured and the data fitted to a two-compartment model, yielding K(m) = 26.2 mM and V(max) = 3.5 nmol/min/cell for GLUT1 and K(m) = 4.3 mM and V(max) = 0.7 nmol/min/cell for GLUT4. Measurement of the abundance of cell surface transporters was accomplished by two independent protocols: photolabeling with the impermeant hexose analog 2-N-4-(1-azi-2,2,2-trifluoroethyl)benzoyl- 1,3-bis(D-mannos-4-yloxy)-2-propylamine and subcellular fractionation of oocytes. Data obtained by either technique revealed that the ratio of plasma membrane GLUT1 to GLUT4 was about 4; this paralleled the relative maximal velocities for hexose transport, indicating that the turnover numbers for the two isoforms were the same. Moreover, measurement of the concentration of exofacially disposed transporters with 2-N-4-(1-azi-2,2,2- trifluoroethyl)benzoyl-1,3-bis(D-mannos-4-yloxy)-2-propylamine allowed calculation of the turnover number to be about 20,000 min-1. These data indicate that, at low substrate concentrations, the catalytic efficiency of GLUT4 is significantly greater than GLUT1. Extrapolation to mammalian systems suggests that GLUT4 is responsible for virtually all of the hexose uptake in insulin-responsive targets, particularly in the presence of hormone.

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Nishimura H, Pallardo FV, Seidner GA, Vannucci S, Simpson I, Birnbaum MJ. Kinetics of GLUT1 and GLUT4 glucose transporters expressed in Xenopus oocytes. Journal of Biological Chemistry. 1993 Jan 1;268(12):8514-8520.