L(-)-10-Formyltetrahydrofolate Is the Cofactor for Glycinamide Ribonucleotide Transformylase from Chicken Liver

Gary K. Smith, Patricia Ann Benkovic, Stephen Benkovic

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Abstract

It is shown that l(-)- 10-formyltetrahydrofolate serves as the cofactor for glycinamide ribonucleotide transformylase from chicken liver. The utilization of l(-)-10-formyl-H4folate was not previously recognized, because L-(+)-10-formyl-H4folate is an excellent competitive inhibitor of the enzyme, Ki = 0.75±0.07µM and historically the transformylase assay was carried out with a mixture of diastereomers. The results are discussed in relation to the utilization of L(+)-5, 10-methenyl-H4folate.

Original languageEnglish (US)
Pages (from-to)4034-4036
Number of pages3
JournalBiochemistry
Volume20
Issue number14
DOIs
Publication statusPublished - Jan 1 1981

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All Science Journal Classification (ASJC) codes

  • Biochemistry

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