L-selectin is required for fMLP- but not C5a-induced margination of neutrophils in pulmonary circulation

Timothy S. Olson, Kai Singbartl, Klaus Ley

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

To study the role of L-selectin in neutrophil (PMN) margination and sequestration in the pulmonary microcirculation, maximally active concentrations of C5a (900 pmol/g) and N-formylmethionyl-leucyl-phenylalanine (fMLP; 0.34 pmol/g) were injected into the jugular vein of wild-type or L-selectin-deficient C57BL/6 mice. In wild-type mice administered C5a or fMLP, 92 ± 1% and 34 ± 9%, respectively, of peripheral blood PMN were trapped mostly in the pulmonary circulation as determined by immunohistochemistry and myeloperoxidase activity. In wild-type mice treated with F(ab′)2 fragments of the L-selectin monoclonal antibody MEL-14 or in L-selectin-deficient mice, C5a-induced neutropenia was not significantly reduced, but the decrease in peripheral PMN in response to fMLP was completely abolished, indicating that L-selectin is necessary for fMLP- but not C5a-induced pulmonary margination. Immunostained lung sections of fMLP- or C5a-treated mice showed sequestered neutrophils in alveolar capillaries with no evidence of neutrophil aggregates. We conclude that chemoattractant-induced PMN margination in the pulmonary circulation can occur by two separate mechanisms, one of which requires L-selectin.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume282
Issue number4 51-4
StatePublished - Jul 2 2002

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L-Selectin
Pulmonary Circulation
Neutrophils
Bronchopulmonary Sequestration
N-Formylmethionine Leucyl-Phenylalanine
Lung
Chemotactic Factors
Jugular Veins
Microcirculation
Neutropenia
Inbred C57BL Mouse
Peroxidase
Immunohistochemistry
Monoclonal Antibodies

All Science Journal Classification (ASJC) codes

  • Physiology
  • Physiology (medical)

Cite this

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title = "L-selectin is required for fMLP- but not C5a-induced margination of neutrophils in pulmonary circulation",
abstract = "To study the role of L-selectin in neutrophil (PMN) margination and sequestration in the pulmonary microcirculation, maximally active concentrations of C5a (900 pmol/g) and N-formylmethionyl-leucyl-phenylalanine (fMLP; 0.34 pmol/g) were injected into the jugular vein of wild-type or L-selectin-deficient C57BL/6 mice. In wild-type mice administered C5a or fMLP, 92 ± 1{\%} and 34 ± 9{\%}, respectively, of peripheral blood PMN were trapped mostly in the pulmonary circulation as determined by immunohistochemistry and myeloperoxidase activity. In wild-type mice treated with F(ab′)2 fragments of the L-selectin monoclonal antibody MEL-14 or in L-selectin-deficient mice, C5a-induced neutropenia was not significantly reduced, but the decrease in peripheral PMN in response to fMLP was completely abolished, indicating that L-selectin is necessary for fMLP- but not C5a-induced pulmonary margination. Immunostained lung sections of fMLP- or C5a-treated mice showed sequestered neutrophils in alveolar capillaries with no evidence of neutrophil aggregates. We conclude that chemoattractant-induced PMN margination in the pulmonary circulation can occur by two separate mechanisms, one of which requires L-selectin.",
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L-selectin is required for fMLP- but not C5a-induced margination of neutrophils in pulmonary circulation. / Olson, Timothy S.; Singbartl, Kai; Ley, Klaus.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 282, No. 4 51-4, 02.07.2002.

Research output: Contribution to journalArticle

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