Label-Free Measurements of Reaction Kinetics Using a Droplet-Based Optofluidic Device

Zhangming Mao, Feng Guo, Yuliang Xie, Yanhui Zhao, Michael Ian Lapsley, Lin Wang, John D. Mai, Francesco Costanzo, Tony Jun Huang

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Label-free measurements of the reaction kinetics of a small sample volume are essential for efficient drug discovery, requiring methods and systems that are rapid, accurate, and cost-effective. Herein, we present an integrated optofluidic system for label-free characterization of reactions in a nanoliter reagent volume. This system contains a droplet-based microfluidic sampling section and an optical fiber–based spectroscopy detection section. By manipulating droplets containing reagents at certain concentrations at different times, quantifiable measurements via absorption spectroscopy can be made in a simple, sensitive, and high-throughput manner. We have demonstrated our system’s capability by performing potency (IC50) assays of an inhibitor in a TEM-1 β-lactamase (enzyme) and nitrocefin (substrate) system. This integrated platform can potentially provide an automated, label-free, and low-cost method for many other assays of reaction kinetics.

Original languageEnglish (US)
Pages (from-to)17-24
Number of pages8
JournalJournal of Laboratory Automation
Volume20
Issue number1
DOIs
StatePublished - Feb 24 2015

All Science Journal Classification (ASJC) codes

  • Computer Science Applications
  • Medical Laboratory Technology

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  • Cite this

    Mao, Z., Guo, F., Xie, Y., Zhao, Y., Lapsley, M. I., Wang, L., Mai, J. D., Costanzo, F., & Huang, T. J. (2015). Label-Free Measurements of Reaction Kinetics Using a Droplet-Based Optofluidic Device. Journal of Laboratory Automation, 20(1), 17-24. https://doi.org/10.1177/2211068214549625