Modification of the asphalt binder is one approach taken to improve Asphalt pavement performance. Rutting is one of the most important factors that could reduce the life of asphalt pavements. Nowadays, the application of nanotechnology to achieve materials that are more resistant is expanding in asphalt pavement that Nano-TiO2 is among the most exciting and promising classes of materials discovered recently. The purpose of this study is laboratory research on the effect of Nano-TiO2 in improving Bitumen property and rutting resistance in Asphalt pavement under dynamic loading. For this purpose, the wheel-tracking test was carried outon ordinary and Nano-TiO2 modified hot mix asphalt samples. The results illustrate that using Nano-TiO2in asphalt binder samples cause to an improvement in rutting depth in comparison with the ordinary mixtures.