Lack of cross-tolerance following heat and cadmium exposure in functional MDCK monolayers

Karol Dokladny, Walker Wharton, Thomas Ma, Pope L. Moseley

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Exposure of monolayers of Madin-Darby canine kidney epithelial (MDCK) cells to a mild heat stimulus induces a state of physiological thermotolerance in which epithelial barrier function is maintained following a second more severe heat stress. We have previously shown that expression of exogenous HSP70 fully mimics the effects of the conditioning heat stress. Exposure of MDCK cells to elevated temperatures or medium containing CdCl2 caused a robust increase in cellular levels of HSP70. Pretreatment of MDCK monolayers with cadmium but not heat caused a small protection of epithelial barrier function against a second challenge with cadmium. In addition, a prior exposure of monolayers to cadmium at levels sufficient to induce HSP70 expression and increased cellular chaperone activity did not afford protection against a subsequent thermal challenge. Therefore multiple stress-specific cellular pathways impinge on the ability of heat shock proteins to induce physiological thermotolerance. Occludin, a component of tight junctions, is induced in MDCK cells engineered to express high levels of exogenous HSP70, potentially accounting for an elevation in baseline resistance. However neither basal levels of occludin, nor alterations in occludin expression, were correlated with epithelial barrier function in MDCK cells either exposed to elevated temperatures or challenged with cadmium.

Original languageEnglish (US)
Pages (from-to)885-894
Number of pages10
JournalJournal of Applied Toxicology
Volume28
Issue number7
DOIs
StatePublished - Oct 1 2008

Fingerprint

Cadmium
Madin Darby Canine Kidney Cells
Canidae
Monolayers
Occludin
Hot Temperature
Kidney
Epithelial Cells
Cadmium Chloride
Temperature
Tight Junctions
Heat-Shock Proteins
Thermotolerance

All Science Journal Classification (ASJC) codes

  • Toxicology

Cite this

Dokladny, Karol ; Wharton, Walker ; Ma, Thomas ; Moseley, Pope L. / Lack of cross-tolerance following heat and cadmium exposure in functional MDCK monolayers. In: Journal of Applied Toxicology. 2008 ; Vol. 28, No. 7. pp. 885-894.
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Lack of cross-tolerance following heat and cadmium exposure in functional MDCK monolayers. / Dokladny, Karol; Wharton, Walker; Ma, Thomas; Moseley, Pope L.

In: Journal of Applied Toxicology, Vol. 28, No. 7, 01.10.2008, p. 885-894.

Research output: Contribution to journalArticle

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