Lack of Glyphosate Resistance Gene Transfer from Roundup Ready® Soybean to Bradyrhizobium japonicum under Field and Laboratory Conditions

Laura Arango Isaza, Katja Opelt, Elke Mattes, Evi Bieber, Heinrich Sandermann, Dieter Ernst, Tobias Wagner, Elwood O. Hatley, Greg Roth, Juan Sanjuan, Hans Martin Fischer, Anton Hartmann

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Abstract

This paper is dedicated to the memory of the late Professor Dr. Heinrich Sandermann, former director of the Institute of Biochemical Plant Pathology, Helmholtz Zentrum Mμnchen A field study was conducted at the Russell E. Larson Agricultural Research Center to determine the effect of transgenic glyphosate-resistant soybean in combination with herbicide (Roundup) application on its endosymbiont Bradyrhizobium japonicum. DNA of bacteroids from isolated nodules was analysed for the presence of the transgenic 5-enolpyruvylshikimate-3-phosphate synthase (CP4-EPSPS) DNA sequence using polymerase chain reaction (PCR). To further assess the likelihood that the EPSPS gene may be transferred from the Roundup Ready® (RR) soybean to B. japonicum, we have examined the natural transformation efficiency of B. japonicum train 110spc4. Analyses of nodules showed the presence of the transgenic EPSPS DNA sequence. In bacteroids that were isolated from nodules of transgenic soybean plants and then cultivated in the presence of glyphosate this sequence could not be detected. This indicates that no stable horizontal gene transfer (HGT) of the EPSPS gene had occurred under field conditions. Under laboratory conditions, no natural transformation was detected in B. japonicum strain 110spc4 in the presence of various amounts of recombinant plasmid DNA. Our results indicate that no natural competence state exists in B. japonicum 110spc4. Results from field and laboratory studies indicate the lack of functional transfer of the CP4-EPSPS gene from glyphosate-tolerant soybean treated with glyphosate to root-associated B. japonicum.

Original languageEnglish (US)
Pages (from-to)595-604
Number of pages10
JournalZeitschrift fur Naturforschung - Section C Journal of Biosciences
Volume66
Issue number11-12
DOIs
StatePublished - Dec 2011

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

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    Isaza, L. A., Opelt, K., Mattes, E., Bieber, E., Sandermann, H., Ernst, D., Wagner, T., Hatley, E. O., Roth, G., Sanjuan, J., Fischer, H. M., & Hartmann, A. (2011). Lack of Glyphosate Resistance Gene Transfer from Roundup Ready® Soybean to Bradyrhizobium japonicum under Field and Laboratory Conditions. Zeitschrift fur Naturforschung - Section C Journal of Biosciences, 66(11-12), 595-604. https://doi.org/10.1515/znc-2011-11-1209