Lack of HPV 16 and 18 detection in serum of colposcopy clinic patients

Divya A. Patel, Elizabeth R. Unger, Heather Walline, Anthony W. Opipari, Daisy R. Lee, Lisa C. Flowers, Mack Ruffin

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background: Persistent infection with high-risk human papillomavirus (HPV) types is necessary for the development of high-grade cervical dysplasia and cervical carcinoma. The presence of HPV DNA in the blood of cervical cancer patients has been reported; however, whether HPV DNA is detectable in the blood of patients with pre-invasive cervical disease is unclear. Objectives: The objectives of this study were to determine if HPV 16 and HPV 18 DNA could be detected in the serum of colposcopy clinic patients, and if serum HPV detection was associated with grade of cervical disease and HPV cofactors. Study design: Samples were selected from a biorepository collected from non-pregnant, HIV-negative women ages 18-69 attending colposcopy clinics at two urban public hospitals. Cervical disease status was based on review of colposcopy, biopsy and cytology findings. Serum HPV DNA detection was conducted using a novel PCR and mass spectroscopy-based assay. Results: Of the 116 adequate serum samples, all (100%) were negative for HPV 16 and HPV 18. Over half (51.7%) of participants had cervical HPV 16 and/or HPV 18 infection. Nearly one-third (31.1%) had high grade, 10.3% had low grade, and 50.9% had no cervical disease. Nearly one-third (28.5%) had ever regularly smoked cigarettes, 70.7% had early onset of sexual intercourse, and 75% had ever used oral contraceptives. Conclusions: In this colposcopy clinic population with a range of clinical characteristics and established HPV cofactors, HPV DNA was undetectable in their serum. Our findings suggest that serum HPV DNA detection is not a cervical cancer screening tool.

Original languageEnglish (US)
Pages (from-to)342-344
Number of pages3
JournalJournal of Clinical Virology
Volume50
Issue number4
DOIs
StatePublished - Apr 1 2011

Fingerprint

Human papillomavirus 18
Colposcopy
Human papillomavirus 16
Serum
DNA
Uterine Cervical Neoplasms
Uterine Cervical Dysplasia
Papillomavirus Infections
Coitus
Public Hospitals
Urban Hospitals
Oral Contraceptives
Early Detection of Cancer
Tobacco Products
Cell Biology
Mass Spectrometry
HIV
Carcinoma
Biopsy
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Virology
  • Infectious Diseases

Cite this

Patel, D. A., Unger, E. R., Walline, H., Opipari, A. W., Lee, D. R., Flowers, L. C., & Ruffin, M. (2011). Lack of HPV 16 and 18 detection in serum of colposcopy clinic patients. Journal of Clinical Virology, 50(4), 342-344. https://doi.org/10.1016/j.jcv.2011.01.002
Patel, Divya A. ; Unger, Elizabeth R. ; Walline, Heather ; Opipari, Anthony W. ; Lee, Daisy R. ; Flowers, Lisa C. ; Ruffin, Mack. / Lack of HPV 16 and 18 detection in serum of colposcopy clinic patients. In: Journal of Clinical Virology. 2011 ; Vol. 50, No. 4. pp. 342-344.
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Patel, DA, Unger, ER, Walline, H, Opipari, AW, Lee, DR, Flowers, LC & Ruffin, M 2011, 'Lack of HPV 16 and 18 detection in serum of colposcopy clinic patients', Journal of Clinical Virology, vol. 50, no. 4, pp. 342-344. https://doi.org/10.1016/j.jcv.2011.01.002

Lack of HPV 16 and 18 detection in serum of colposcopy clinic patients. / Patel, Divya A.; Unger, Elizabeth R.; Walline, Heather; Opipari, Anthony W.; Lee, Daisy R.; Flowers, Lisa C.; Ruffin, Mack.

In: Journal of Clinical Virology, Vol. 50, No. 4, 01.04.2011, p. 342-344.

Research output: Contribution to journalArticle

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Patel DA, Unger ER, Walline H, Opipari AW, Lee DR, Flowers LC et al. Lack of HPV 16 and 18 detection in serum of colposcopy clinic patients. Journal of Clinical Virology. 2011 Apr 1;50(4):342-344. https://doi.org/10.1016/j.jcv.2011.01.002