Lactic acid production in a mixed-culture biofilm reactor

Ali Demirci, A. L. Pometto, K. E. Johnson

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Novel solid supports, consisting of polypropylene blended with various agricultural materials (pp composite), were evaluated as supports for pure- and mixed-culture continuous lactic acid fermentations in biofilm reactors. Streptomyces viridosporus T7A (ATCC 39115) was used to form a biofilm, and Lactobacillus casei subsp. rhamnosus (ATCC 11443) was used for lactic acid production. For mixed-culture fermentations, a 15-day continuous fermentation of S. viridosporus was performed initially to establish the biofilm. The culture medium was then inoculated with L. casei subsp. rhamnosus. For pure- culture fermentation, L. casei subsp. rhamnosus was inoculated directly into the reactors containing sterile pp composite chips. The biofilm reactors containing various pp composite chips were compared with a biofilm reactor containing pure polypropylene chips and with a reactor containing a suspension culture. Continuous fermentation was started, and each flow rate (0.06 to 1.92 ml/min) was held constant for 24 h; steady state was achieved after 10 h. Lactic acid production was determined throughout the 24-h period by high-performance liquid chromatography. Production rates that were two to five times faster than those of the suspension culture (control) were observed for the pure- and mixed-culture bioreactors. Both lactic acid production rates and lactic acid concentrations in the culture medium were consistently higher in mixed-culture than in pure-culture fermentations. Biofilm formation on the chips was detected at harvest by chip clumping and Gram staining.

Original languageEnglish (US)
Pages (from-to)203-207
Number of pages5
JournalApplied and Environmental Microbiology
Volume59
Issue number1
StatePublished - Jan 1 1993

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mixed culture
Biofilms
biofilm
lactic acid
Fermentation
Lactic Acid
Lactobacillus casei
Streptomyces viridosporus
Lactobacillus rhamnosus
acid
fermentation
continuous fermentation
Polypropylenes
polypropylenes
Culture Media
Suspensions
culture media
lactic fermentation
composite materials
Streptomyces

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

Demirci, Ali ; Pometto, A. L. ; Johnson, K. E. / Lactic acid production in a mixed-culture biofilm reactor. In: Applied and Environmental Microbiology. 1993 ; Vol. 59, No. 1. pp. 203-207.
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Lactic acid production in a mixed-culture biofilm reactor. / Demirci, Ali; Pometto, A. L.; Johnson, K. E.

In: Applied and Environmental Microbiology, Vol. 59, No. 1, 01.01.1993, p. 203-207.

Research output: Contribution to journalArticle

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