Laminar and temporal expression dynamics of coding and noncoding RNAs in the mouse neocortex

Sofia Fertuzinhos, Mingfeng Li, Yuka Imamura Kawasawa, Vedrana Ivic, Daniel Franjic, Darshani Singh, Michael Crair, Nenad Šestan

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The hallmark of the cerebral neocortex is its organization into six layers, each containing a characteristic set of cell types and synaptic connections. The transcriptional events involved in laminar development and function still remain elusive. Here, we employed deep sequencing of mRNA and small RNA species to gain insights into transcriptional differences among layers and their temporal dynamics during postnatal development of the mouse primary somatosensory neocortex. We identify a number ofcoding and noncoding transcripts with specific spatiotemporal expression and splicing patterns. We also identify signature trajectories and gene coexpression networks associated with distinct biological processes and transcriptional overlap between these processes. Finally, we provide data that allow the study of potential miRNA and mRNA interactions. Overall, this study provides an integrated view of the laminar and temporal expression dynamics of coding and noncoding transcripts in the mouse neocortex and a resource for studies of neurodevelopment and transcriptome.

Original languageEnglish (US)
Pages (from-to)938-950
Number of pages13
JournalCell Reports
Volume6
Issue number5
DOIs
StatePublished - Jan 1 2014

Fingerprint

Untranslated RNA
Neocortex
Messenger RNA
MicroRNAs
Biological Phenomena
High-Throughput Nucleotide Sequencing
Genes
Gene Regulatory Networks
Trajectories
RNA
Transcriptome

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Fertuzinhos, Sofia ; Li, Mingfeng ; Kawasawa, Yuka Imamura ; Ivic, Vedrana ; Franjic, Daniel ; Singh, Darshani ; Crair, Michael ; Šestan, Nenad. / Laminar and temporal expression dynamics of coding and noncoding RNAs in the mouse neocortex. In: Cell Reports. 2014 ; Vol. 6, No. 5. pp. 938-950.
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Fertuzinhos, S, Li, M, Kawasawa, YI, Ivic, V, Franjic, D, Singh, D, Crair, M & Šestan, N 2014, 'Laminar and temporal expression dynamics of coding and noncoding RNAs in the mouse neocortex', Cell Reports, vol. 6, no. 5, pp. 938-950. https://doi.org/10.1016/j.celrep.2014.01.036

Laminar and temporal expression dynamics of coding and noncoding RNAs in the mouse neocortex. / Fertuzinhos, Sofia; Li, Mingfeng; Kawasawa, Yuka Imamura; Ivic, Vedrana; Franjic, Daniel; Singh, Darshani; Crair, Michael; Šestan, Nenad.

In: Cell Reports, Vol. 6, No. 5, 01.01.2014, p. 938-950.

Research output: Contribution to journalArticle

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