Langerhans cells arise from monocytes in vivo

Florent Ginhoux, Frank Tacke, Veronique Angeli, Milena Bogunovic, Martine Loubeau, Xu Ming Dai, E. Richard Stanley, Gwendalyn J. Randolph, Miriam Merad

Research output: Contribution to journalArticle

464 Citations (Scopus)

Abstract

Langerhans cells (LCs) are the only dendritic cells of the epidermis and constitute the first immunological barrier against pathogens and environmental insults. The factors regulating LC homeostasis remain elusive and the direct circulating LC precursor has not yet been identified in vivo. Here we report an absence of LCs in mice deficient in the receptor for colony-stimulating factor 1 (CSF-1) in steady-state conditions. Using bone marrow chimeric mice, we have established that CSF-1 receptor-deficient hematopoietic precursors failed to reconstitute the LC pool in inflamed skin. Furthermore, monocytes with high expression of the monocyte marker Gr-1 (also called Ly-6c/G) were specifically recruited to the inflamed skin, proliferated locally and differentiated into LCs. These results identify Gr-1hi monocytes as the direct precursors for LCs in vivo and establish the importance of the CSF-1 receptor in this process.

Original languageEnglish (US)
Pages (from-to)265-273
Number of pages9
JournalNature Immunology
Volume7
Issue number3
DOIs
StatePublished - Mar 1 2006

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Langerhans Cells
Monocytes
Macrophage Colony-Stimulating Factor
Colony-Stimulating Factor Receptors
Skin
Epidermis
Dendritic Cells
Homeostasis
Bone Marrow

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

Ginhoux, F., Tacke, F., Angeli, V., Bogunovic, M., Loubeau, M., Dai, X. M., ... Merad, M. (2006). Langerhans cells arise from monocytes in vivo. Nature Immunology, 7(3), 265-273. https://doi.org/10.1038/ni1307
Ginhoux, Florent ; Tacke, Frank ; Angeli, Veronique ; Bogunovic, Milena ; Loubeau, Martine ; Dai, Xu Ming ; Stanley, E. Richard ; Randolph, Gwendalyn J. ; Merad, Miriam. / Langerhans cells arise from monocytes in vivo. In: Nature Immunology. 2006 ; Vol. 7, No. 3. pp. 265-273.
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Ginhoux, F, Tacke, F, Angeli, V, Bogunovic, M, Loubeau, M, Dai, XM, Stanley, ER, Randolph, GJ & Merad, M 2006, 'Langerhans cells arise from monocytes in vivo', Nature Immunology, vol. 7, no. 3, pp. 265-273. https://doi.org/10.1038/ni1307

Langerhans cells arise from monocytes in vivo. / Ginhoux, Florent; Tacke, Frank; Angeli, Veronique; Bogunovic, Milena; Loubeau, Martine; Dai, Xu Ming; Stanley, E. Richard; Randolph, Gwendalyn J.; Merad, Miriam.

In: Nature Immunology, Vol. 7, No. 3, 01.03.2006, p. 265-273.

Research output: Contribution to journalArticle

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Ginhoux F, Tacke F, Angeli V, Bogunovic M, Loubeau M, Dai XM et al. Langerhans cells arise from monocytes in vivo. Nature Immunology. 2006 Mar 1;7(3):265-273. https://doi.org/10.1038/ni1307