Large double-stranded RNA molecules in Phaseolus vulgaris L. are not associated with cytoplasmic male sterility

Sally Mackenzie, D. R. Pring, M. J. Bassett

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Two large double-stranded RNA molecules, 15 and 16 kilobases, were detected in cytoplasmic male sterile (CMS) Phaseolus vulgaris by agarose gel electrophoresis. A number of smaller RNA molecules were observed in 'Sprite', a maintainer line, and recurrent backcrossing of CMS P. vulgarisx'Sprite' resulted in a combined electrophoretic pattern of the two large and numerous small RNA molecules. The large RNA molecules were seed and pollen-transmissible, but were not transmitted by grafting. The RNAs were present in revertant and restored lines derived from CMS-Sprite and therefore were not associated with the cytoplasmic male sterile trait.

Original languageEnglish (US)
Pages (from-to)59-63
Number of pages5
JournalTheoretical and Applied Genetics
Volume76
Issue number1
DOIs
StatePublished - Jul 1 1988

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science
  • Genetics

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