Large plasma-membrane depolarization precedes rapid blue-light-induced growth inhibition in cucumber

Edgar P. Spalding, Daniel J. Cosgrove

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

Blue-light (BL)-induced suppression of elongation of etiolated Cucumis sativus L. hypocotyls began after a 30-s lag time, which was halved by increasing the fluence rate from 10 to 100 μmol·m-2·s-1. Prior to the growth suppression, the plasma-membrane of the irradiated cells depolarized by as much as 100 mV, then returned within 2-3 min to near its initial value. The potential difference measured with surface electrodes changed with an identical time course but opposite polarity. The lag time for the change in surface potential showed an inverse dependence on fluence rate, similar to the lag for the growth inhibition. Green light and red light caused neither the electrical response nor the rapid inhibition of growth. The depolarization by BL did not propagate to nonirradiated regions and exhibited a refractory period of about 10 min following a BL pulse. Fluenceresponse relationships for the electrical and growth responses provide correlational evidence that the plasma-membrane depolarization reflects an event in the transduction chain of this light-growth response.

Original languageEnglish (US)
Pages (from-to)407-410
Number of pages4
JournalPlanta
Volume178
Issue number3
DOIs
StatePublished - Jun 1 1989

Fingerprint

Cucumis sativus
blue light
growth retardation
cucumbers
plasma membrane
Cell Membrane
Light
Growth
red light
electrodes
hypocotyls
Methyl Green
Hypocotyl
Electrodes
cells

All Science Journal Classification (ASJC) codes

  • Plant Science

Cite this

@article{e518b0dce6554cbcae0d89522a74c0d3,
title = "Large plasma-membrane depolarization precedes rapid blue-light-induced growth inhibition in cucumber",
abstract = "Blue-light (BL)-induced suppression of elongation of etiolated Cucumis sativus L. hypocotyls began after a 30-s lag time, which was halved by increasing the fluence rate from 10 to 100 μmol·m-2·s-1. Prior to the growth suppression, the plasma-membrane of the irradiated cells depolarized by as much as 100 mV, then returned within 2-3 min to near its initial value. The potential difference measured with surface electrodes changed with an identical time course but opposite polarity. The lag time for the change in surface potential showed an inverse dependence on fluence rate, similar to the lag for the growth inhibition. Green light and red light caused neither the electrical response nor the rapid inhibition of growth. The depolarization by BL did not propagate to nonirradiated regions and exhibited a refractory period of about 10 min following a BL pulse. Fluenceresponse relationships for the electrical and growth responses provide correlational evidence that the plasma-membrane depolarization reflects an event in the transduction chain of this light-growth response.",
author = "Spalding, {Edgar P.} and Cosgrove, {Daniel J.}",
year = "1989",
month = "6",
day = "1",
doi = "10.1007/BF00391869",
language = "English (US)",
volume = "178",
pages = "407--410",
journal = "Planta",
issn = "0032-0935",
publisher = "Springer Verlag",
number = "3",

}

Large plasma-membrane depolarization precedes rapid blue-light-induced growth inhibition in cucumber. / Spalding, Edgar P.; Cosgrove, Daniel J.

In: Planta, Vol. 178, No. 3, 01.06.1989, p. 407-410.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Large plasma-membrane depolarization precedes rapid blue-light-induced growth inhibition in cucumber

AU - Spalding, Edgar P.

AU - Cosgrove, Daniel J.

PY - 1989/6/1

Y1 - 1989/6/1

N2 - Blue-light (BL)-induced suppression of elongation of etiolated Cucumis sativus L. hypocotyls began after a 30-s lag time, which was halved by increasing the fluence rate from 10 to 100 μmol·m-2·s-1. Prior to the growth suppression, the plasma-membrane of the irradiated cells depolarized by as much as 100 mV, then returned within 2-3 min to near its initial value. The potential difference measured with surface electrodes changed with an identical time course but opposite polarity. The lag time for the change in surface potential showed an inverse dependence on fluence rate, similar to the lag for the growth inhibition. Green light and red light caused neither the electrical response nor the rapid inhibition of growth. The depolarization by BL did not propagate to nonirradiated regions and exhibited a refractory period of about 10 min following a BL pulse. Fluenceresponse relationships for the electrical and growth responses provide correlational evidence that the plasma-membrane depolarization reflects an event in the transduction chain of this light-growth response.

AB - Blue-light (BL)-induced suppression of elongation of etiolated Cucumis sativus L. hypocotyls began after a 30-s lag time, which was halved by increasing the fluence rate from 10 to 100 μmol·m-2·s-1. Prior to the growth suppression, the plasma-membrane of the irradiated cells depolarized by as much as 100 mV, then returned within 2-3 min to near its initial value. The potential difference measured with surface electrodes changed with an identical time course but opposite polarity. The lag time for the change in surface potential showed an inverse dependence on fluence rate, similar to the lag for the growth inhibition. Green light and red light caused neither the electrical response nor the rapid inhibition of growth. The depolarization by BL did not propagate to nonirradiated regions and exhibited a refractory period of about 10 min following a BL pulse. Fluenceresponse relationships for the electrical and growth responses provide correlational evidence that the plasma-membrane depolarization reflects an event in the transduction chain of this light-growth response.

UR - http://www.scopus.com/inward/record.url?scp=0024958929&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024958929&partnerID=8YFLogxK

U2 - 10.1007/BF00391869

DO - 10.1007/BF00391869

M3 - Article

C2 - 11537725

AN - SCOPUS:0024958929

VL - 178

SP - 407

EP - 410

JO - Planta

JF - Planta

SN - 0032-0935

IS - 3

ER -