Latent murine γ-herpesvirus infection is established in activated B cells, dendritic cells, and macrophages

Emilio Flaño, S. Mazher Husain, Jeffery Sample, David L. Woodland, Marcia A. Blackman

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Abstract

Intranasal infection of mice with the murine γ-herpesvirus MHV-68 results in an acute lytic infection in the lung, followed by the establishment of lifelong latency. Development of an infectious mononucleosis-like syndrome correlates with the establishment of latency and is characterized by splenomegaly and the appearance of activated CD8+ T cells in the peripheral blood. Interestingly, a large population of activated CD8+ T cells in the peripheral blood express the Vβ4+ element in their TCR. In this report we show that MHV-68 latency in the spleen after intranasal infection is harbored in three APC types: B cells, macrophages, and dendritic cells. Surprisingly, since latency has not previously been described in dendritic cells, these cells harbored the highest frequency of latent virus. Among B cells, latency was preferentially associated with activated B cells expressing the phenotype of germinal center B cells, thus formally linking the previously reported association of latency gene expression and germinal centers to germinal center B cells. Germinal center formation, however, was not required for the establishment of latency. Significantly, although three cell types were latently infected, the ability to stimulate Vβ4+ CD8+ T cell hybridomas was limited to latently infected, activated B cells.

Original languageEnglish (US)
Pages (from-to)1074-1081
Number of pages8
JournalJournal of Immunology
Volume165
Issue number2
StatePublished - Jul 15 2000

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All Science Journal Classification (ASJC) codes

  • Immunology

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