Leaky resistance and the conditions for the existence of lytic bacteriophage

Waqas N. Chaudhry, Maroš Pleška, Nilang N. Shah, Howard Weiss, Ingrid C. McCall, Justin R. Meyer, Animesh Gupta, Călin C. Guet, Bruce R. Levin

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10 Scopus citations

Abstract

In experimental cultures, when bacteria are mixed with lytic (virulent) bacteriophage, bacterial cells resistant to the phage commonly emerge and become the dominant population of bacteria. Following the ascent of resistant mutants, the densities of bacteria in these simple communities become limited by resources rather than the phage. Despite the evolution of resistant hosts, upon which the phage cannot replicate, the lytic phage population is most commonly maintained in an apparently stable state with the resistant bacteria. Several mechanisms have been put forward to account for this result. Here we report the results of population dynamic/evolution experiments with a virulent mutant of phage Lambda, λ VIR , and Escherichia coli in serial transfer cultures. We show that, following the ascent of λ VIR -resistant bacteria, λ VIR is maintained in the majority of cases in maltose-limited minimal media and in all cases in nutrient-rich broth. Using mathematical models and experiments, we show that the dominant mechanism responsible for maintenance of λ VIR in these resource-limited populations dominated by resistant E. coli is a high rate of either phenotypic or genetic transition from resistance to susceptibility—a hitherto undemonstrated mechanism we term "leaky resistance." We discuss the implications of leaky resistance to our understanding of the conditions for the maintenance of phage in populations of bacteria—their “existence conditions.”.

Original languageEnglish (US)
Article numbere2005971
JournalPLoS biology
Volume16
Issue number8
DOIs
StatePublished - Aug 16 2018

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Agricultural and Biological Sciences(all)

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    Chaudhry, W. N., Pleška, M., Shah, N. N., Weiss, H., McCall, I. C., Meyer, J. R., Gupta, A., Guet, C. C., & Levin, B. R. (2018). Leaky resistance and the conditions for the existence of lytic bacteriophage. PLoS biology, 16(8), [e2005971]. https://doi.org/10.1371/journal.pbio.2005971