Leukocyte β 2-adrenergic receptor expression in response to resistance exercise

Maren S. Fragala, William J. Kraemer, Andrea M. Mastro, Craig R. Denegar, Jeff S. Volek, Keijo Häkkinen, Jeffrey M. Anderson, Elaine C. Lee, Carl M. Maresh

Research output: Contribution to journalArticlepeer-review

28 Scopus citations


Purpose: Epinephrine and norepinephrine mediate interactions between the neuroendocrine and the immune systems to alter immune cell activity. Although both systems respond to exercise stress, less is known about how they interact in response to such stress. The purpose of this investigation was to examine β 2-adrenergic receptor (β 2-ADR) expression on circulating leukocytes to an acute bout of resistance exercise in men and women. Methods: Resistance-trained men (n = 8; mean ± SD age = 24.63 ± 5.07 yr, body mass index = 26.09 ± 2.21 kg·m -2) and women (n = 7; age = 22.13 ± 3.09 yr, body mass index = 22.63 ± 2.03 kg·m -2) performed an acute resistance exercise protocol (six sets of five-repetition maximum heavy squats) and a control test (i.e., identical conditions with no exercise) in a balanced, randomized order. Using a within-subject design, β 2-ADR expressions on circulating leukocytes were evaluated with flow cytometry, and plasma epinephrine and norepinephrine were evaluated with high-performance liquid chromatography. Results: Plasma epinephrine and norepinephrine increased during the exercise bout and returned to baseline during recovery. β 2-ADR expression on monocytes was elevated in anticipation of the exercise protocol. β 2-ADR expression on monocytes and granulocytes decreased during the exercise. β 2-ADR expression on lymphocytes was elevated during the recovery time points. Conclusions: In conclusion, β 2-ADR expression on leukocyte subpopulations changes in response to acute heavy resistance exercise protocol. The present findings provide insights into the potential temporal interactions between the neuroendocrine and the immune systems in response to the physiological stress of acute heavy resistance exercise in men and women.

Original languageEnglish (US)
Pages (from-to)1422-1432
Number of pages11
JournalMedicine and science in sports and exercise
Issue number8
StatePublished - Aug 2011

All Science Journal Classification (ASJC) codes

  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation


Dive into the research topics of 'Leukocyte β <sub>2</sub>-adrenergic receptor expression in response to resistance exercise'. Together they form a unique fingerprint.

Cite this