Leukotrap, a device for white cell poor platelets quality control studies In vitro and In vivo

M. Sternbach, J. Champagne, Witold Rybka, M. Paquin

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Most febrile transfusion reactions are due to leucoagglutinins. Cutter's Leukotrap platelet pooling bag has a distal conic pouch for depleting the platelets of white blood cells by centrifugation. We tested 33 Leukotraps each containing six platelet units in vitro and 32 in vivo. The mean in vitro platelet count was 3.7 ± 0.5 × 1011 platelets before, and 3.0 ± 0.5 × 1011 after spinning, representing a platelet recovery of 80.2 ± 9.6% Mean white blood cells were 3.8 ± 0.6 × 108 before, and 0.6 ± 0.1 × 108 after centrifugation, this constituting a white cell removal of 83.5 ± 7.7%. pH ranged from 7.37 for 24-h platelets to 7.19 for 96-h platelets. 24-h after platelet pooling, all Leukotraps were sterile. Platelet aggregation with physiologic agents showed little change compared to individual platelet units. Glucose ranged between 418 and 336 mg/dL, pCO2 between 27.8 and 19.1 mmHg, but pO2 dropped drastically from 74.8 mmHg to 11.6 mmHg. Hypotonic osmotic recovery was satisfactory. In vivo studies were carried out with pooled, leucocyte-poor platelets which were transfused to six bone marrow transplant patients with no splenomegaly or septicemia at the outset. These patients had all demonstrated febrile transfusion reactions to standard donor units. The mean platelet increment was 16.8 × 109/L. A single febrile transfusion reaction witnessed in one patient, was accompanied by an adequate platelet response. Hence Leukotrap is a useful clinical tool for reducing febrile transfusion reactions related to white blood cells.

Original languageEnglish (US)
Pages (from-to)57-62
Number of pages6
JournalTransfusion Science
Volume10
Issue number1
DOIs
StatePublished - Jan 1 1989

Fingerprint

Quality Control
Blood Platelets
Equipment and Supplies
Leukocytes
Fever
Centrifugation
In Vitro Techniques
Splenomegaly
Platelet Count
Platelet Aggregation
Sepsis
Bone Marrow
Tissue Donors
Transplants
Glucose
Transfusion Reaction

All Science Journal Classification (ASJC) codes

  • Immunology
  • Hematology

Cite this

Sternbach, M. ; Champagne, J. ; Rybka, Witold ; Paquin, M. / Leukotrap, a device for white cell poor platelets quality control studies In vitro and In vivo. In: Transfusion Science. 1989 ; Vol. 10, No. 1. pp. 57-62.
@article{8ef5836ff9d34fb0a52817f4b9e10f89,
title = "Leukotrap, a device for white cell poor platelets quality control studies In vitro and In vivo",
abstract = "Most febrile transfusion reactions are due to leucoagglutinins. Cutter's Leukotrap platelet pooling bag has a distal conic pouch for depleting the platelets of white blood cells by centrifugation. We tested 33 Leukotraps each containing six platelet units in vitro and 32 in vivo. The mean in vitro platelet count was 3.7 ± 0.5 × 1011 platelets before, and 3.0 ± 0.5 × 1011 after spinning, representing a platelet recovery of 80.2 ± 9.6{\%} Mean white blood cells were 3.8 ± 0.6 × 108 before, and 0.6 ± 0.1 × 108 after centrifugation, this constituting a white cell removal of 83.5 ± 7.7{\%}. pH ranged from 7.37 for 24-h platelets to 7.19 for 96-h platelets. 24-h after platelet pooling, all Leukotraps were sterile. Platelet aggregation with physiologic agents showed little change compared to individual platelet units. Glucose ranged between 418 and 336 mg/dL, pCO2 between 27.8 and 19.1 mmHg, but pO2 dropped drastically from 74.8 mmHg to 11.6 mmHg. Hypotonic osmotic recovery was satisfactory. In vivo studies were carried out with pooled, leucocyte-poor platelets which were transfused to six bone marrow transplant patients with no splenomegaly or septicemia at the outset. These patients had all demonstrated febrile transfusion reactions to standard donor units. The mean platelet increment was 16.8 × 109/L. A single febrile transfusion reaction witnessed in one patient, was accompanied by an adequate platelet response. Hence Leukotrap is a useful clinical tool for reducing febrile transfusion reactions related to white blood cells.",
author = "M. Sternbach and J. Champagne and Witold Rybka and M. Paquin",
year = "1989",
month = "1",
day = "1",
doi = "10.1016/0955-3886(89)90009-X",
language = "English (US)",
volume = "10",
pages = "57--62",
journal = "Transfusion and Apheresis Science",
issn = "1473-0502",
publisher = "Elsevier Limited",
number = "1",

}

Leukotrap, a device for white cell poor platelets quality control studies In vitro and In vivo. / Sternbach, M.; Champagne, J.; Rybka, Witold; Paquin, M.

In: Transfusion Science, Vol. 10, No. 1, 01.01.1989, p. 57-62.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Leukotrap, a device for white cell poor platelets quality control studies In vitro and In vivo

AU - Sternbach, M.

AU - Champagne, J.

AU - Rybka, Witold

AU - Paquin, M.

PY - 1989/1/1

Y1 - 1989/1/1

N2 - Most febrile transfusion reactions are due to leucoagglutinins. Cutter's Leukotrap platelet pooling bag has a distal conic pouch for depleting the platelets of white blood cells by centrifugation. We tested 33 Leukotraps each containing six platelet units in vitro and 32 in vivo. The mean in vitro platelet count was 3.7 ± 0.5 × 1011 platelets before, and 3.0 ± 0.5 × 1011 after spinning, representing a platelet recovery of 80.2 ± 9.6% Mean white blood cells were 3.8 ± 0.6 × 108 before, and 0.6 ± 0.1 × 108 after centrifugation, this constituting a white cell removal of 83.5 ± 7.7%. pH ranged from 7.37 for 24-h platelets to 7.19 for 96-h platelets. 24-h after platelet pooling, all Leukotraps were sterile. Platelet aggregation with physiologic agents showed little change compared to individual platelet units. Glucose ranged between 418 and 336 mg/dL, pCO2 between 27.8 and 19.1 mmHg, but pO2 dropped drastically from 74.8 mmHg to 11.6 mmHg. Hypotonic osmotic recovery was satisfactory. In vivo studies were carried out with pooled, leucocyte-poor platelets which were transfused to six bone marrow transplant patients with no splenomegaly or septicemia at the outset. These patients had all demonstrated febrile transfusion reactions to standard donor units. The mean platelet increment was 16.8 × 109/L. A single febrile transfusion reaction witnessed in one patient, was accompanied by an adequate platelet response. Hence Leukotrap is a useful clinical tool for reducing febrile transfusion reactions related to white blood cells.

AB - Most febrile transfusion reactions are due to leucoagglutinins. Cutter's Leukotrap platelet pooling bag has a distal conic pouch for depleting the platelets of white blood cells by centrifugation. We tested 33 Leukotraps each containing six platelet units in vitro and 32 in vivo. The mean in vitro platelet count was 3.7 ± 0.5 × 1011 platelets before, and 3.0 ± 0.5 × 1011 after spinning, representing a platelet recovery of 80.2 ± 9.6% Mean white blood cells were 3.8 ± 0.6 × 108 before, and 0.6 ± 0.1 × 108 after centrifugation, this constituting a white cell removal of 83.5 ± 7.7%. pH ranged from 7.37 for 24-h platelets to 7.19 for 96-h platelets. 24-h after platelet pooling, all Leukotraps were sterile. Platelet aggregation with physiologic agents showed little change compared to individual platelet units. Glucose ranged between 418 and 336 mg/dL, pCO2 between 27.8 and 19.1 mmHg, but pO2 dropped drastically from 74.8 mmHg to 11.6 mmHg. Hypotonic osmotic recovery was satisfactory. In vivo studies were carried out with pooled, leucocyte-poor platelets which were transfused to six bone marrow transplant patients with no splenomegaly or septicemia at the outset. These patients had all demonstrated febrile transfusion reactions to standard donor units. The mean platelet increment was 16.8 × 109/L. A single febrile transfusion reaction witnessed in one patient, was accompanied by an adequate platelet response. Hence Leukotrap is a useful clinical tool for reducing febrile transfusion reactions related to white blood cells.

UR - http://www.scopus.com/inward/record.url?scp=0024391562&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024391562&partnerID=8YFLogxK

U2 - 10.1016/0955-3886(89)90009-X

DO - 10.1016/0955-3886(89)90009-X

M3 - Article

VL - 10

SP - 57

EP - 62

JO - Transfusion and Apheresis Science

JF - Transfusion and Apheresis Science

SN - 1473-0502

IS - 1

ER -