Levels of immunoreactive glycine conjugated bile acids in health and hepatobiliary disease

L. M. Demers, G. W. Hepner

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

A sensitive radioimmunoassay for cholylglycine, chenodeoxycholylglycine, deoxycholylglycine, and sulfolithocholylglycine was established using antibodies obtained from rabbits injected with albumin conjugates of these bile acids. Glycine conjugated bile acid levels were measured in sera from 25 control subjects and 110 patients who had hepatic disease (alcoholic cirrhosis, hepatitis, cholestasis, and hepatic malignancy). Sulfolithocholylglycine was elevated in the sera of all 110 patients with hepatic disease. Cholylglycine was within normal range in only three. Chenodeoxycholylglycine was elevated in most sera of patients who had hepatitis, cholestasis, or hepatic malignancy. It was normal in most sera of patients who had alcoholic cirrhosis, suggesting that chendeoxycholic acid may be subject to further biotransformations in these patients. Deoxycholylglycine was elevated in a minority of patients, none of whom had cholestasis. The data suggest that serum bile acids, particularly sulfolithocholylglycine, are a highly sensitive index for hepatic dysfunction.

Original languageEnglish (US)
Pages (from-to)831-839
Number of pages9
JournalAmerican journal of clinical pathology
Volume66
Issue number5
DOIs
StatePublished - Jan 1 1976

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

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