Light-emitting-diode-based multispectral photoacoustic computed tomography system

Sumit Agrawal, Christopher Fadden, Ajay Dangi, Xinyi Yang, Hussain Albahrani, Neilesh Frings, Sara Heidari Zadi, Sri Rajasekhar Kothapalli

Research output: Contribution to journalArticle

Abstract

Photoacoustic computed tomography (PACT) has been widely explored for non-ionizing functional and molecular imaging of humans and small animals. In order for light to penetrate deep inside tissue, a bulky and high-cost tunable laser is typically used. Light-emitting diodes (LEDs) have recently emerged as cost-effective and portable alternative illumination sources for photoacoustic imaging. In this study, we have developed a portable, low-cost, five-dimensional (x, y, z, t, λ) PACT system using multi-wavelength LED excitation to enable similar functional and molecular imaging capabilities as standard tunable lasers. Four LED arrays and a linear ultrasound transducer detector array are housed in a hollow cylindrical geometry that rotates 360 degrees to allow multiple projections through the subject of interest placed inside the cylinder. The structural, functional, and molecular imaging capabilities of the LED–PACT system are validated using various tissue-mimicking phantom studies. The axial, lateral, and elevational resolutions of the system at 2.3 cm depth are estimated as 0.12 mm, 0.3 mm, and 2.1 mm, respectively. Spectrally unmixed photoacoustic contrasts from tubes filled with oxy-and deoxy-hemoglobin, indocyanine green, methylene blue, and melanin molecules demonstrate the multispectral molecular imaging capabilities of the system. Human-finger-mimicking phantoms made of a bone and blood tubes show structural and functional oxygen saturation imaging capabilities. Together, these results demonstrate the potential of the proposed LED-based, low-cost, portable PACT system for pre-clinical and clinical applications.

Original languageEnglish (US)
Article number4861
JournalSensors (Switzerland)
Volume19
Issue number22
DOIs
StatePublished - Nov 2 2019

Fingerprint

Photoacoustic effect
Molecular imaging
Molecular Imaging
Tomography
Light emitting diodes
light emitting diodes
tomography
Light
Costs and Cost Analysis
tunable lasers
Laser tuning
Costs
tubes
costs
melanin
Lasers
Tissue
Melanin
methylene blue
hemoglobin

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Atomic and Molecular Physics, and Optics
  • Instrumentation
  • Electrical and Electronic Engineering

Cite this

Agrawal, Sumit ; Fadden, Christopher ; Dangi, Ajay ; Yang, Xinyi ; Albahrani, Hussain ; Frings, Neilesh ; Zadi, Sara Heidari ; Kothapalli, Sri Rajasekhar. / Light-emitting-diode-based multispectral photoacoustic computed tomography system. In: Sensors (Switzerland). 2019 ; Vol. 19, No. 22.
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Agrawal, S, Fadden, C, Dangi, A, Yang, X, Albahrani, H, Frings, N, Zadi, SH & Kothapalli, SR 2019, 'Light-emitting-diode-based multispectral photoacoustic computed tomography system', Sensors (Switzerland), vol. 19, no. 22, 4861. https://doi.org/10.3390/s19224861

Light-emitting-diode-based multispectral photoacoustic computed tomography system. / Agrawal, Sumit; Fadden, Christopher; Dangi, Ajay; Yang, Xinyi; Albahrani, Hussain; Frings, Neilesh; Zadi, Sara Heidari; Kothapalli, Sri Rajasekhar.

In: Sensors (Switzerland), Vol. 19, No. 22, 4861, 02.11.2019.

Research output: Contribution to journalArticle

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Agrawal S, Fadden C, Dangi A, Yang X, Albahrani H, Frings N et al. Light-emitting-diode-based multispectral photoacoustic computed tomography system. Sensors (Switzerland). 2019 Nov 2;19(22). 4861. https://doi.org/10.3390/s19224861