Light-mediated hydrogen generation in photosystem I: Attachment of a naphthoquinone-molecular wire-Pt nanoparticle to the A1A and A 1B sites

Michael J. Gorka, Jonas Schartner, Art Van Der Est, Matthias Rögner, John H. Golbeck

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The molecular wire-appended naphthoquinone 1-[15-(3-methyl-1,4- naphthoquinone-2-yl)]pentadecyl disulfide [(NQ(CH2) 15S)2] has been incorporated into the A1A and A1B sites of Photosystem I (PS I) in the menB variant of Synechocystis sp. PCC 6803. Transient electron paramagnetic resonance studies show that the naphthoquinone headgroup displaces plastoquinone-9 from the A 1A (and likely A1B) sites to a large extent. When a Pt nanoparticle is attached to the molecular wire by reductive cleavage of the disulfide and reaction with the resulting thiol, the PS I-NQ(CH 2)15S-Pt nanoconstruct evolves dihydrogen at a rate of 67.3 μmol of H2 (mg of Chl)-1 h-1 [3.4 e- (PS I)-1 s-1] after illumination for 1 h at pH 6.4. No dihydrogen is detected if wild-type PS I, which does not incorporate the quinone, is used or if either (NQ(CH2)15S)2 or the Pt nanoparticle is absent. Time-resolved optical studies of the PS I-NQ(CH2)15S-Pt nanoconstruct show that the lifetimes of the forward electron transfer to and reverse electron transfer from the iron-sulfur clusters are the same as in native PS I. Thus, electrons are not shuttled directly from the quinone to the Pt nanoparticle during either forward or reverse electron transfer. It is found that the rate of dihydrogen evolution in the PS I-NQ(CH2)15S-Pt nanoconstruct depends strongly on the concentration the sacrificial electron donor cytochrome c6. These observations can be explained if the iron-sulfur clusters are involved in stabilizing the electron; the ∼50 ms residence time of the electron on FA or FB is sufficiently long to allow cytochrome c 6 to reduce P700+, thereby eliminating the recombination channel. In the absence of P700+, slow electron transfer through the molecular wire to the Pt catalyst can occur, and hence, H2 evolution is observed.

Original languageEnglish (US)
Pages (from-to)2295-2306
Number of pages12
JournalBiochemistry
Volume53
Issue number14
DOIs
StatePublished - Apr 15 2014

Fingerprint

Naphthoquinones
Photosystem I Protein Complex
Nanoparticles
Hydrogen
Wire
Electrons
Light
Sulfur
Disulfides
Cytochromes c6
Iron
Plastoquinone
Synechocystis
Vitamin K 3
Electron Spin Resonance Spectroscopy
Cytochromes c
Lighting
Sulfhydryl Compounds
Genetic Recombination
Paramagnetic resonance

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

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title = "Light-mediated hydrogen generation in photosystem I: Attachment of a naphthoquinone-molecular wire-Pt nanoparticle to the A1A and A 1B sites",
abstract = "The molecular wire-appended naphthoquinone 1-[15-(3-methyl-1,4- naphthoquinone-2-yl)]pentadecyl disulfide [(NQ(CH2) 15S)2] has been incorporated into the A1A and A1B sites of Photosystem I (PS I) in the menB variant of Synechocystis sp. PCC 6803. Transient electron paramagnetic resonance studies show that the naphthoquinone headgroup displaces plastoquinone-9 from the A 1A (and likely A1B) sites to a large extent. When a Pt nanoparticle is attached to the molecular wire by reductive cleavage of the disulfide and reaction with the resulting thiol, the PS I-NQ(CH 2)15S-Pt nanoconstruct evolves dihydrogen at a rate of 67.3 μmol of H2 (mg of Chl)-1 h-1 [3.4 e- (PS I)-1 s-1] after illumination for 1 h at pH 6.4. No dihydrogen is detected if wild-type PS I, which does not incorporate the quinone, is used or if either (NQ(CH2)15S)2 or the Pt nanoparticle is absent. Time-resolved optical studies of the PS I-NQ(CH2)15S-Pt nanoconstruct show that the lifetimes of the forward electron transfer to and reverse electron transfer from the iron-sulfur clusters are the same as in native PS I. Thus, electrons are not shuttled directly from the quinone to the Pt nanoparticle during either forward or reverse electron transfer. It is found that the rate of dihydrogen evolution in the PS I-NQ(CH2)15S-Pt nanoconstruct depends strongly on the concentration the sacrificial electron donor cytochrome c6. These observations can be explained if the iron-sulfur clusters are involved in stabilizing the electron; the ∼50 ms residence time of the electron on FA or FB is sufficiently long to allow cytochrome c 6 to reduce P700+, thereby eliminating the recombination channel. In the absence of P700+, slow electron transfer through the molecular wire to the Pt catalyst can occur, and hence, H2 evolution is observed.",
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Light-mediated hydrogen generation in photosystem I : Attachment of a naphthoquinone-molecular wire-Pt nanoparticle to the A1A and A 1B sites. / Gorka, Michael J.; Schartner, Jonas; Van Der Est, Art; Rögner, Matthias; Golbeck, John H.

In: Biochemistry, Vol. 53, No. 14, 15.04.2014, p. 2295-2306.

Research output: Contribution to journalArticle

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T1 - Light-mediated hydrogen generation in photosystem I

T2 - Attachment of a naphthoquinone-molecular wire-Pt nanoparticle to the A1A and A 1B sites

AU - Gorka, Michael J.

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AU - Van Der Est, Art

AU - Rögner, Matthias

AU - Golbeck, John H.

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N2 - The molecular wire-appended naphthoquinone 1-[15-(3-methyl-1,4- naphthoquinone-2-yl)]pentadecyl disulfide [(NQ(CH2) 15S)2] has been incorporated into the A1A and A1B sites of Photosystem I (PS I) in the menB variant of Synechocystis sp. PCC 6803. Transient electron paramagnetic resonance studies show that the naphthoquinone headgroup displaces plastoquinone-9 from the A 1A (and likely A1B) sites to a large extent. When a Pt nanoparticle is attached to the molecular wire by reductive cleavage of the disulfide and reaction with the resulting thiol, the PS I-NQ(CH 2)15S-Pt nanoconstruct evolves dihydrogen at a rate of 67.3 μmol of H2 (mg of Chl)-1 h-1 [3.4 e- (PS I)-1 s-1] after illumination for 1 h at pH 6.4. No dihydrogen is detected if wild-type PS I, which does not incorporate the quinone, is used or if either (NQ(CH2)15S)2 or the Pt nanoparticle is absent. Time-resolved optical studies of the PS I-NQ(CH2)15S-Pt nanoconstruct show that the lifetimes of the forward electron transfer to and reverse electron transfer from the iron-sulfur clusters are the same as in native PS I. Thus, electrons are not shuttled directly from the quinone to the Pt nanoparticle during either forward or reverse electron transfer. It is found that the rate of dihydrogen evolution in the PS I-NQ(CH2)15S-Pt nanoconstruct depends strongly on the concentration the sacrificial electron donor cytochrome c6. These observations can be explained if the iron-sulfur clusters are involved in stabilizing the electron; the ∼50 ms residence time of the electron on FA or FB is sufficiently long to allow cytochrome c 6 to reduce P700+, thereby eliminating the recombination channel. In the absence of P700+, slow electron transfer through the molecular wire to the Pt catalyst can occur, and hence, H2 evolution is observed.

AB - The molecular wire-appended naphthoquinone 1-[15-(3-methyl-1,4- naphthoquinone-2-yl)]pentadecyl disulfide [(NQ(CH2) 15S)2] has been incorporated into the A1A and A1B sites of Photosystem I (PS I) in the menB variant of Synechocystis sp. PCC 6803. Transient electron paramagnetic resonance studies show that the naphthoquinone headgroup displaces plastoquinone-9 from the A 1A (and likely A1B) sites to a large extent. When a Pt nanoparticle is attached to the molecular wire by reductive cleavage of the disulfide and reaction with the resulting thiol, the PS I-NQ(CH 2)15S-Pt nanoconstruct evolves dihydrogen at a rate of 67.3 μmol of H2 (mg of Chl)-1 h-1 [3.4 e- (PS I)-1 s-1] after illumination for 1 h at pH 6.4. No dihydrogen is detected if wild-type PS I, which does not incorporate the quinone, is used or if either (NQ(CH2)15S)2 or the Pt nanoparticle is absent. Time-resolved optical studies of the PS I-NQ(CH2)15S-Pt nanoconstruct show that the lifetimes of the forward electron transfer to and reverse electron transfer from the iron-sulfur clusters are the same as in native PS I. Thus, electrons are not shuttled directly from the quinone to the Pt nanoparticle during either forward or reverse electron transfer. It is found that the rate of dihydrogen evolution in the PS I-NQ(CH2)15S-Pt nanoconstruct depends strongly on the concentration the sacrificial electron donor cytochrome c6. These observations can be explained if the iron-sulfur clusters are involved in stabilizing the electron; the ∼50 ms residence time of the electron on FA or FB is sufficiently long to allow cytochrome c 6 to reduce P700+, thereby eliminating the recombination channel. In the absence of P700+, slow electron transfer through the molecular wire to the Pt catalyst can occur, and hence, H2 evolution is observed.

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