Light-regulated overexpression of an Arabidopsis phytochrome A gene in rice alters plant architecture and increases grain yield

Ajay K. Garg, Ruairidh Sawers, Haiyang Wang, Ju Kon Kim, Joseph M. Walker, Thomas P. Brutnell, Mandayam V. Parthasarathy, Richard D. Vierstra, Ray J. Wu

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

The phytochromes are a family of red/far-red light absorbing photoreceptors that control plant developmental and metabolic processes in response to changes in the light environment. We report here the overexpression of Arabidopsis thaliana PHYTOCHROME A (PHYA) gene in a commercially important indica rice variety (Oryza sativa L. Pusa Basmati-1). The expression of the transgene was driven by the light-regulated and tissue-specific rice rbcS promoter. Several independent homozygous sixth generation (T5) transgenic lines were characterized and shown to accumulate relatively high levels of PHYA protein in the light. Under both far-red and red light, PHYA-overexpressing lines showed inhibition of the coleoptile extension in comparison to non-transgenic seedlings. Furthermore, compared with non-transgenic rice plants, mature transgenic plants showed significant reduction in plant height, internode length and internode diameter (including differences in cell size and number), and produced an increased number of panicles per plant. Under greenhouse conditions, rice grain yield was 6-21% higher in three PHYA-overexpressing lines than in non-transgenic plants. These results demonstrate the potential of manipulating light signal-transduction pathways to minimize the problems of lodging in basmati/aromatic rice and to enhance grain productivity.

Original languageEnglish (US)
Pages (from-to)627-636
Number of pages10
JournalPlanta
Volume223
Issue number4
DOIs
StatePublished - Mar 1 2006

Fingerprint

Phytochrome A
plant architecture
phytochrome
Arabidopsis
grain yield
Light
rice
Genes
genes
internodes
Plant Photoreceptors
phototransduction
Phytochrome
Light Signal Transduction
lodging
far-red light
coleoptiles
red light
mature plants
photoreceptors

All Science Journal Classification (ASJC) codes

  • Genetics
  • Plant Science

Cite this

Garg, Ajay K. ; Sawers, Ruairidh ; Wang, Haiyang ; Kim, Ju Kon ; Walker, Joseph M. ; Brutnell, Thomas P. ; Parthasarathy, Mandayam V. ; Vierstra, Richard D. ; Wu, Ray J. / Light-regulated overexpression of an Arabidopsis phytochrome A gene in rice alters plant architecture and increases grain yield. In: Planta. 2006 ; Vol. 223, No. 4. pp. 627-636.
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Garg, AK, Sawers, R, Wang, H, Kim, JK, Walker, JM, Brutnell, TP, Parthasarathy, MV, Vierstra, RD & Wu, RJ 2006, 'Light-regulated overexpression of an Arabidopsis phytochrome A gene in rice alters plant architecture and increases grain yield', Planta, vol. 223, no. 4, pp. 627-636. https://doi.org/10.1007/s00425-005-0101-3

Light-regulated overexpression of an Arabidopsis phytochrome A gene in rice alters plant architecture and increases grain yield. / Garg, Ajay K.; Sawers, Ruairidh; Wang, Haiyang; Kim, Ju Kon; Walker, Joseph M.; Brutnell, Thomas P.; Parthasarathy, Mandayam V.; Vierstra, Richard D.; Wu, Ray J.

In: Planta, Vol. 223, No. 4, 01.03.2006, p. 627-636.

Research output: Contribution to journalArticle

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AU - Garg, Ajay K.

AU - Sawers, Ruairidh

AU - Wang, Haiyang

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AU - Walker, Joseph M.

AU - Brutnell, Thomas P.

AU - Parthasarathy, Mandayam V.

AU - Vierstra, Richard D.

AU - Wu, Ray J.

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