Light regulation of protein dimerization and kinase activity in living cells using photocaged rapamycin and engineered FKBP

Andrei V. Karginov, Yan Zou, David Shirvanyants, Pradeep Kota, Nikolay V. Dokholyan, Douglas D. Young, Klaus M. Hahn, Alexander Deiters

Research output: Contribution to journalArticlepeer-review

87 Scopus citations

Abstract

We developed a new system for light-induced protein dimerization in living cells using a photocaged analogue of rapamycin together with an engineered rapamycin binding domain. Using focal adhesion kinase as a target, we demonstrated successful light-mediated regulation of protein interaction and localization in living cells. Modification of this approach enabled light-triggered activation of a protein kinase and initiation of kinase-induced phenotypic changes in vivo.

Original languageEnglish (US)
Pages (from-to)420-423
Number of pages4
JournalJournal of the American Chemical Society
Volume133
Issue number3
DOIs
StatePublished - Jan 26 2011

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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