Light scattering properties of kidney epithelial cells and nuclei

Elina A. Vitol, Timothy P. Kurzweg, Bahram Nabet

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

Enlargement of mammalian cells nuclei due to the cancerous inflammation can be detected early through noninvasive optical techniques. We report on the results of cellular experiments, aimed towards the development of a fiber optic endoscopic probe used for precancerous detection of Barrett's esophagus. We previously presented white light scattering results from tissue phantoms (polystyrene polybead microspheres). In this paper, we discuss light scattering properties of epithelial MDCK (Madine-Darby Canine Kidney) cells and cell nuclei suspensions. A bifurcated optical fiber is used for experimental illumination and signal detection. The resulting scattering spectra from the cells do not exhibit the predicted Mie theory oscillatory behavior inherent to ideally spherical scatterers, such as polystyrene microspheres. However, we are able to demonstrate that the Fourier transform spectra of the cell suspensions are well correlated with the Fourier transform spectra of cell nuclei, concluding that the dominate scatterer in the backscattering region is the nucleus. This correlation experimentally illustrates that in the backscattering region, the cell nuclei are the main scatterer in the cells of the incident light.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
DOIs
StatePublished - May 8 2006
EventOptical Biopsy VI - San Jose, CA, United States
Duration: Jan 24 2006Jan 24 2006

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6091
ISSN (Print)1605-7422

Conference

ConferenceOptical Biopsy VI
CountryUnited States
CitySan Jose, CA
Period1/24/061/24/06

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Vitol, E. A., Kurzweg, T. P., & Nabet, B. (2006). Light scattering properties of kidney epithelial cells and nuclei. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE [609108] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6091). https://doi.org/10.1117/12.644863