"Light-tagged" bacteriophage as a diagnostic tool for the detection of phytopathogens

David A. Schofield, Carolee T. Bull, Isael Rubio, W. Patrick Wechter, Caroline Westwater, Ian J. Molineux

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9 Scopus citations

Abstract

Detection of the phytopathogen Pseudomonas cannabina pv alisalensis, the causal agent of bacterial blight of crucifers is essential for managing this disease. A phage-based diagnostic assay was developed that detects and identifies P. cannabina pv alisalensis from cultures and diseased plant specimens. A recombinant "light-tagged" reporter phage was generated by integrating the luxAB genes into the P. cannabina pv alisalensis phage PBSPCA1 genome. PBSPCA1::luxAB is viable, stable and detects P. cannabina pv alisalensis within minutes and with high sensitivity by conferring a bioluminescent signal. Detection is dependent on cell viability since cells treated with a bactericidal disinfectant are unable to elicit a signal. Importantly, the reporter phage detects P. cannabina pv alisalensis from diseased plant specimens indicating the potential of the diagnostic for disease identification. The reporter phage displays promise for the rapid and specific diagnostic detection of cultivated isolates, and infected plant specimens.

Original languageEnglish (US)
JournalBioengineered
Volume4
Issue number1
DOIs
Publication statusPublished - Jan 1 2013

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All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Schofield, D. A., Bull, C. T., Rubio, I., Patrick Wechter, W., Westwater, C., & Molineux, I. J. (2013). "Light-tagged" bacteriophage as a diagnostic tool for the detection of phytopathogens. Bioengineered, 4(1). https://doi.org/10.4161/bioe.22159