"Light-tagged" bacteriophage as a diagnostic tool for the detection of phytopathogens

David A. Schofield, Carolee Theresa Bull, Isael Rubio, W. Patrick Wechter, Caroline Westwater, Ian J. Molineux

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Detection of the phytopathogen Pseudomonas cannabina pv alisalensis, the causal agent of bacterial blight of crucifers is essential for managing this disease. A phage-based diagnostic assay was developed that detects and identifies P. cannabina pv alisalensis from cultures and diseased plant specimens. A recombinant "light-tagged" reporter phage was generated by integrating the luxAB genes into the P. cannabina pv alisalensis phage PBSPCA1 genome. PBSPCA1::luxAB is viable, stable and detects P. cannabina pv alisalensis within minutes and with high sensitivity by conferring a bioluminescent signal. Detection is dependent on cell viability since cells treated with a bactericidal disinfectant are unable to elicit a signal. Importantly, the reporter phage detects P. cannabina pv alisalensis from diseased plant specimens indicating the potential of the diagnostic for disease identification. The reporter phage displays promise for the rapid and specific diagnostic detection of cultivated isolates, and infected plant specimens.

Original languageEnglish (US)
JournalBioengineered
Volume4
Issue number1
DOIs
StatePublished - Jan 1 2013

Fingerprint

Bacteriophages
Light
Plant Diseases
Genes
Disinfectants
Pseudomonas
Assays
Cell Survival
Cells
Genome

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Schofield, D. A., Bull, C. T., Rubio, I., Patrick Wechter, W., Westwater, C., & Molineux, I. J. (2013). "Light-tagged" bacteriophage as a diagnostic tool for the detection of phytopathogens. Bioengineered, 4(1). https://doi.org/10.4161/bioe.22159
Schofield, David A. ; Bull, Carolee Theresa ; Rubio, Isael ; Patrick Wechter, W. ; Westwater, Caroline ; Molineux, Ian J. / "Light-tagged" bacteriophage as a diagnostic tool for the detection of phytopathogens. In: Bioengineered. 2013 ; Vol. 4, No. 1.
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Schofield, DA, Bull, CT, Rubio, I, Patrick Wechter, W, Westwater, C & Molineux, IJ 2013, '"Light-tagged" bacteriophage as a diagnostic tool for the detection of phytopathogens', Bioengineered, vol. 4, no. 1. https://doi.org/10.4161/bioe.22159

"Light-tagged" bacteriophage as a diagnostic tool for the detection of phytopathogens. / Schofield, David A.; Bull, Carolee Theresa; Rubio, Isael; Patrick Wechter, W.; Westwater, Caroline; Molineux, Ian J.

In: Bioengineered, Vol. 4, No. 1, 01.01.2013.

Research output: Contribution to journalArticle

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