Lignin peroxidase from fungi: Phanerochaete chrysosporium

T. Kent Kirk, Ming Tien, Philip J. Kersten, B. Kalyanaraman, Kenneth E. Hammel, Roberta L. Farrell

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Lignin peroxidase of Phanerochaete chrysosporium can be produced and purified relatively easily. Described here is production and separation of six isoenzymes, using a commonly studied wild-type strain. The fungus is grown on a chemically defined nitrogen-limiting medium in stationary or agitated flasks, the extracellular broth containing the lignin peroxidases is concentrated, and the isoenzymes are separated and purified by anionexchange chromatography. The isoenzymes have similar physical and catalytic properties but somewhat different kinetic properties. They are encoded at least in part by different structural genes. Lignin peroxidascs, in the presence of H2O2, oxidize their aromatic substrates by one electron to cation radicals, which undergo diverse reactions of ionic and radical nature. Purification and characterization of lignin peroxidases from other lignin-degrading fungi have also been reported.

Original languageEnglish (US)
Pages (from-to)159-171
Number of pages13
JournalMethods in enzymology
Volume188
Issue numberC
DOIs
StatePublished - Jan 1 1990

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Phanerochaete
Fungi
Isoenzymes
Lignin
Chromatography
Purification
Cations
Nitrogen
Genes
Electrons
Kinetics
Substrates
lignin peroxidase

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Kent Kirk, T., Tien, M., Kersten, P. J., Kalyanaraman, B., Hammel, K. E., & Farrell, R. L. (1990). Lignin peroxidase from fungi: Phanerochaete chrysosporium. Methods in enzymology, 188(C), 159-171. https://doi.org/10.1016/0076-6879(90)88029-A
Kent Kirk, T. ; Tien, Ming ; Kersten, Philip J. ; Kalyanaraman, B. ; Hammel, Kenneth E. ; Farrell, Roberta L. / Lignin peroxidase from fungi : Phanerochaete chrysosporium. In: Methods in enzymology. 1990 ; Vol. 188, No. C. pp. 159-171.
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Kent Kirk, T, Tien, M, Kersten, PJ, Kalyanaraman, B, Hammel, KE & Farrell, RL 1990, 'Lignin peroxidase from fungi: Phanerochaete chrysosporium', Methods in enzymology, vol. 188, no. C, pp. 159-171. https://doi.org/10.1016/0076-6879(90)88029-A

Lignin peroxidase from fungi : Phanerochaete chrysosporium. / Kent Kirk, T.; Tien, Ming; Kersten, Philip J.; Kalyanaraman, B.; Hammel, Kenneth E.; Farrell, Roberta L.

In: Methods in enzymology, Vol. 188, No. C, 01.01.1990, p. 159-171.

Research output: Contribution to journalArticle

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Kent Kirk T, Tien M, Kersten PJ, Kalyanaraman B, Hammel KE, Farrell RL. Lignin peroxidase from fungi: Phanerochaete chrysosporium. Methods in enzymology. 1990 Jan 1;188(C):159-171. https://doi.org/10.1016/0076-6879(90)88029-A