Lignin peroxidase of Phanerochaete chrysosporium

Ming Tien, T. Kent Kirk

Research output: Contribution to journalArticle

1162 Citations (Scopus)

Abstract

Several procedures have been described for growing Phanerochaete chrysosporiurn for ligninase production. These procedures differ somewhat in the medium formulation and types of growth vessels: (1) shallow stationary cultures, (2) agitated liquid cultures, and (3) rotating biological contactors (RBCs; disk fermenters). The recently developed use of agitated culture for production of ligninase permits easier “scale up.” Although ligninase can be produced in agitated flask cultures, the reliable use of stirred tank fermenters awaits further development, which is ongoing in several laboratories. This chapter describes the production of ligninase in shallow stationary cultures and in agitated cultures. Shallow stationary cultures (10 ml) are grown in rubber-stoppered, 125-ml Edenmyer flasks at 39 ° under 100% oxygen. The stationary cultures give somewhat more reliable and reproducible results than the agitated cultures.

Original languageEnglish (US)
Pages (from-to)238-249
Number of pages12
JournalMethods in Enzymology
Volume161
Issue numberC
DOIs
StatePublished - Jan 1 1988

Fingerprint

Phanerochaete
Fermenters
Rubber
Oxygen
lignin peroxidase
ligninase
Liquids
Growth

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Tien, Ming ; Kirk, T. Kent. / Lignin peroxidase of Phanerochaete chrysosporium. In: Methods in Enzymology. 1988 ; Vol. 161, No. C. pp. 238-249.
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Lignin peroxidase of Phanerochaete chrysosporium. / Tien, Ming; Kirk, T. Kent.

In: Methods in Enzymology, Vol. 161, No. C, 01.01.1988, p. 238-249.

Research output: Contribution to journalArticle

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