Limited genetic diversity in the PvK12 Kelch protein in Plasmodium vivax isolates from Southeast Asia

Meilian Wang, Faiza Amber Siddiqui, Qi Fan, Enjie Luo, Yaming Cao, Liwang Cui

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background: Artemisinin resistance in Plasmodium falciparum has emerged as a major threat for malaria control and elimination worldwide. Mutations in the Kelch propeller domain of PfK13 are the only known molecular markers for artemisinin resistance in this parasite. Over 100 non-synonymous mutations have been identified in PfK13 from various malaria endemic regions. This study aimed to investigate the genetic diversity of PvK12, the Plasmodium vivax ortholog of PfK13, in parasite populations from Southeast Asia, where artemisinin resistance in P. falciparum has emerged. Methods: The PvK12 sequences in 120 P. vivax isolates collected from Thailand (22), Myanmar (32) and China (66) between 2004 and 2008 were obtained and 353 PvK12 sequences from worldwide populations were retrieved for further analysis. Results: These PvK12 sequences revealed a very low level of genetic diversity (π = 0.00003) with only three single nucleotide polymorphisms (SNPs). Of these three SNPs, only G581R is nonsynonymous. The synonymous mutation S88S is present in 3% (1/32) of the Myanmar samples, while G704G and G581R are present in 1.5% (1/66) and 3% (2/66) of the samples from China, respectively. None of the mutations observed in the P. vivax samples were associated with artemisinin resistance in P. falciparum. Furthermore, analysis of 473 PvK12 sequences from twelve worldwide P. vivax populations confirmed the very limited polymorphism in this gene and detected only five distinct haplotypes. Conclusions: The PvK12 sequences from global P. vivax populations displayed very limited genetic diversity indicating low levels of baseline polymorphisms of PvK12 in these areas.

Original languageEnglish (US)
Pages (from-to)1-10
Number of pages10
JournalMalaria journal
Volume15
Issue number1
DOIs
StatePublished - Nov 8 2016

Fingerprint

Plasmodium vivax
Southeastern Asia
Plasmodium falciparum
Myanmar
Proteins
Mutation
Population
Malaria
Single Nucleotide Polymorphism
China
Parasites
Thailand
Haplotypes
artemisinine
Genes

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Infectious Diseases

Cite this

Wang, Meilian ; Siddiqui, Faiza Amber ; Fan, Qi ; Luo, Enjie ; Cao, Yaming ; Cui, Liwang. / Limited genetic diversity in the PvK12 Kelch protein in Plasmodium vivax isolates from Southeast Asia. In: Malaria journal. 2016 ; Vol. 15, No. 1. pp. 1-10.
@article{677ac8ff0abd4933ae90c298216f3bc6,
title = "Limited genetic diversity in the PvK12 Kelch protein in Plasmodium vivax isolates from Southeast Asia",
abstract = "Background: Artemisinin resistance in Plasmodium falciparum has emerged as a major threat for malaria control and elimination worldwide. Mutations in the Kelch propeller domain of PfK13 are the only known molecular markers for artemisinin resistance in this parasite. Over 100 non-synonymous mutations have been identified in PfK13 from various malaria endemic regions. This study aimed to investigate the genetic diversity of PvK12, the Plasmodium vivax ortholog of PfK13, in parasite populations from Southeast Asia, where artemisinin resistance in P. falciparum has emerged. Methods: The PvK12 sequences in 120 P. vivax isolates collected from Thailand (22), Myanmar (32) and China (66) between 2004 and 2008 were obtained and 353 PvK12 sequences from worldwide populations were retrieved for further analysis. Results: These PvK12 sequences revealed a very low level of genetic diversity (π = 0.00003) with only three single nucleotide polymorphisms (SNPs). Of these three SNPs, only G581R is nonsynonymous. The synonymous mutation S88S is present in 3{\%} (1/32) of the Myanmar samples, while G704G and G581R are present in 1.5{\%} (1/66) and 3{\%} (2/66) of the samples from China, respectively. None of the mutations observed in the P. vivax samples were associated with artemisinin resistance in P. falciparum. Furthermore, analysis of 473 PvK12 sequences from twelve worldwide P. vivax populations confirmed the very limited polymorphism in this gene and detected only five distinct haplotypes. Conclusions: The PvK12 sequences from global P. vivax populations displayed very limited genetic diversity indicating low levels of baseline polymorphisms of PvK12 in these areas.",
author = "Meilian Wang and Siddiqui, {Faiza Amber} and Qi Fan and Enjie Luo and Yaming Cao and Liwang Cui",
year = "2016",
month = "11",
day = "8",
doi = "10.1186/s12936-016-1583-0",
language = "English (US)",
volume = "15",
pages = "1--10",
journal = "Malaria Journal",
issn = "1475-2875",
publisher = "BioMed Central",
number = "1",

}

Limited genetic diversity in the PvK12 Kelch protein in Plasmodium vivax isolates from Southeast Asia. / Wang, Meilian; Siddiqui, Faiza Amber; Fan, Qi; Luo, Enjie; Cao, Yaming; Cui, Liwang.

In: Malaria journal, Vol. 15, No. 1, 08.11.2016, p. 1-10.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Limited genetic diversity in the PvK12 Kelch protein in Plasmodium vivax isolates from Southeast Asia

AU - Wang, Meilian

AU - Siddiqui, Faiza Amber

AU - Fan, Qi

AU - Luo, Enjie

AU - Cao, Yaming

AU - Cui, Liwang

PY - 2016/11/8

Y1 - 2016/11/8

N2 - Background: Artemisinin resistance in Plasmodium falciparum has emerged as a major threat for malaria control and elimination worldwide. Mutations in the Kelch propeller domain of PfK13 are the only known molecular markers for artemisinin resistance in this parasite. Over 100 non-synonymous mutations have been identified in PfK13 from various malaria endemic regions. This study aimed to investigate the genetic diversity of PvK12, the Plasmodium vivax ortholog of PfK13, in parasite populations from Southeast Asia, where artemisinin resistance in P. falciparum has emerged. Methods: The PvK12 sequences in 120 P. vivax isolates collected from Thailand (22), Myanmar (32) and China (66) between 2004 and 2008 were obtained and 353 PvK12 sequences from worldwide populations were retrieved for further analysis. Results: These PvK12 sequences revealed a very low level of genetic diversity (π = 0.00003) with only three single nucleotide polymorphisms (SNPs). Of these three SNPs, only G581R is nonsynonymous. The synonymous mutation S88S is present in 3% (1/32) of the Myanmar samples, while G704G and G581R are present in 1.5% (1/66) and 3% (2/66) of the samples from China, respectively. None of the mutations observed in the P. vivax samples were associated with artemisinin resistance in P. falciparum. Furthermore, analysis of 473 PvK12 sequences from twelve worldwide P. vivax populations confirmed the very limited polymorphism in this gene and detected only five distinct haplotypes. Conclusions: The PvK12 sequences from global P. vivax populations displayed very limited genetic diversity indicating low levels of baseline polymorphisms of PvK12 in these areas.

AB - Background: Artemisinin resistance in Plasmodium falciparum has emerged as a major threat for malaria control and elimination worldwide. Mutations in the Kelch propeller domain of PfK13 are the only known molecular markers for artemisinin resistance in this parasite. Over 100 non-synonymous mutations have been identified in PfK13 from various malaria endemic regions. This study aimed to investigate the genetic diversity of PvK12, the Plasmodium vivax ortholog of PfK13, in parasite populations from Southeast Asia, where artemisinin resistance in P. falciparum has emerged. Methods: The PvK12 sequences in 120 P. vivax isolates collected from Thailand (22), Myanmar (32) and China (66) between 2004 and 2008 were obtained and 353 PvK12 sequences from worldwide populations were retrieved for further analysis. Results: These PvK12 sequences revealed a very low level of genetic diversity (π = 0.00003) with only three single nucleotide polymorphisms (SNPs). Of these three SNPs, only G581R is nonsynonymous. The synonymous mutation S88S is present in 3% (1/32) of the Myanmar samples, while G704G and G581R are present in 1.5% (1/66) and 3% (2/66) of the samples from China, respectively. None of the mutations observed in the P. vivax samples were associated with artemisinin resistance in P. falciparum. Furthermore, analysis of 473 PvK12 sequences from twelve worldwide P. vivax populations confirmed the very limited polymorphism in this gene and detected only five distinct haplotypes. Conclusions: The PvK12 sequences from global P. vivax populations displayed very limited genetic diversity indicating low levels of baseline polymorphisms of PvK12 in these areas.

UR - http://www.scopus.com/inward/record.url?scp=84994376906&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84994376906&partnerID=8YFLogxK

U2 - 10.1186/s12936-016-1583-0

DO - 10.1186/s12936-016-1583-0

M3 - Article

VL - 15

SP - 1

EP - 10

JO - Malaria Journal

JF - Malaria Journal

SN - 1475-2875

IS - 1

ER -