Limited global diversity of the Plasmodium vivax merozoite surface protein 4 gene

Chaturong Putaporntip, Somchai Jongwutiwes, Marcelo U. Ferreira, Hiroji Kanbara, Rachanee Udomsangpetch, Liwang Cui

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Merozoite surface proteins (MSPs) of the malaria parasites are major candidates for vaccine development targeting asexual blood stages. However, the diverse antigenic repertoire of these antigens that induce strain-specific protective immunity in human is a major challenge for vaccine design and often determines the efficacy of a vaccine. Here we further assessed the genetic diversity of Plasmodium vivax MSP4 (PvMSP4) protein using 195 parasite samples collected mostly from Thailand, Indonesia and Brazil. Overall, PvMSP4 is highly conserved with only eight amino acid substitutions. The majority of the haplotype diversity was restricted to the two short tetrapeptide repeat arrays in exon 1 and 2, respectively. Selection and neutrality tests indicated that exon 1 and the entire coding region of PvMSP4 were under purifying selection. Despite the limited nucleotide polymorphism of PvMSP4, significant genetic differentiation among the three major parasite populations was detected. Moreover, microgeographical heterogeneity was also evident in the parasite populations from different endemic areas of Thailand.

Original languageEnglish (US)
Pages (from-to)821-826
Number of pages6
JournalInfection, Genetics and Evolution
Volume9
Issue number5
DOIs
StatePublished - Sep 2009

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Microbiology (medical)
  • Infectious Diseases

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