Linkage between proton binding and folding in RNA: Implications for RNA catalysis

P. C. Bevilacqua, T. S. Brown, D. Chadalavada, J. Lecomte, E. Moody, S. I. Nakano

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Small ribozymes use their nucleobases to catalyse phosphodiester bond cleavage. The hepatitis delta virus ribozyme employs C75 as a general acid to protonate the 5′-bridging oxygen leaving group, and to accomplish this task efficiently, it shifts its pKa towards neutrality. Simulations and thermodynamic experiments implicate linkage between folding and protonation in nucleobase pKa shifting. Even small oligonucleotides are shown to fold in a highly co-operative manner, although they do so in a context-specific fashion. Linkage between protonation and co-operativity of folding may drive pKa shifting and provide for enhanced function in RNA.

Original languageEnglish (US)
Pages (from-to)466-470
Number of pages5
JournalBiochemical Society transactions
Volume33
Issue number3
DOIs
StatePublished - Jun 1 2005

Fingerprint

RNA Folding
Catalytic RNA
Protonation
Catalysis
Protons
RNA
Hepatitis Delta Virus
Viruses
Thermodynamics
Oligonucleotides
Oxygen
Acids
Experiments

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

Bevilacqua, P. C. ; Brown, T. S. ; Chadalavada, D. ; Lecomte, J. ; Moody, E. ; Nakano, S. I. / Linkage between proton binding and folding in RNA : Implications for RNA catalysis. In: Biochemical Society transactions. 2005 ; Vol. 33, No. 3. pp. 466-470.
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Linkage between proton binding and folding in RNA : Implications for RNA catalysis. / Bevilacqua, P. C.; Brown, T. S.; Chadalavada, D.; Lecomte, J.; Moody, E.; Nakano, S. I.

In: Biochemical Society transactions, Vol. 33, No. 3, 01.06.2005, p. 466-470.

Research output: Contribution to journalArticle

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