TY - JOUR
T1 - Linkage of an ABCC transporter to a single QTL that controls Ostrinia nubilalis larval resistance to the Bacillus thuringiensis Cry1Fa toxin
AU - Coates, Brad S.
AU - Siegfried, Blair D.
N1 - Funding Information:
This research was a joint contribution from the United States Department of Agriculture (USDA), Agricultural Research Service (ARS) (CRIS Project 3625-22000-017-00), and the Iowa Agriculture and Home Economics Experiment Station, Ames, IA (Project 3543). Funding also was provided by a USDA, National Institute of Food and Agriculture , Biotechnology Risk Assessment Grant (NIFA-BRAG; Award Number 2010-33522-21673 ). Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA or the University of Nebraska for its use. USDA is an equal opportunity provider and employer.
Publisher Copyright:
© 2015.
PY - 2015/8/1
Y1 - 2015/8/1
N2 - Field evolved resistance of insect populations to Bacillus thuringiensis (Bt) crystalline (Cry) toxins expressed by crop plants has resulted in reduced control of insect feeding damage to field crops, and threatens the sustainability of Bt transgenic technologies. A single quantitative trait locus (QTL) that determines resistance in Ostrinia nubilalis larvae capable of surviving on reproductive stage transgenic corn that express the Bt Cry1Fa toxin was previously mapped to linkage group 12 (LG12) in a backcross pedigree. Fine mapping with high-throughput single nucleotide polymorphism (SNP) anchor markers, a candidate ABC transporter (. abcc2) marker, and de novo mutations predicted from a genotyping-by-sequencing (GBS) data redefined a 268.8 cM LG12. The single QTL on LG12 spanned an approximate 46.1 cM region, in which marker 02302.286 and abcc2 were ≤2.81 cM, and the GBS marker 697 was an estimated 1.89 cM distant from the causal genetic factor. This positional mapping data showed that an O. nubilalis genome region encoding an abcc2 transporter is in proximity to a single QTL involved in the inheritance of Cry1F resistance, and will assist in the future identification the mutation(s) involved with this phenotype.
AB - Field evolved resistance of insect populations to Bacillus thuringiensis (Bt) crystalline (Cry) toxins expressed by crop plants has resulted in reduced control of insect feeding damage to field crops, and threatens the sustainability of Bt transgenic technologies. A single quantitative trait locus (QTL) that determines resistance in Ostrinia nubilalis larvae capable of surviving on reproductive stage transgenic corn that express the Bt Cry1Fa toxin was previously mapped to linkage group 12 (LG12) in a backcross pedigree. Fine mapping with high-throughput single nucleotide polymorphism (SNP) anchor markers, a candidate ABC transporter (. abcc2) marker, and de novo mutations predicted from a genotyping-by-sequencing (GBS) data redefined a 268.8 cM LG12. The single QTL on LG12 spanned an approximate 46.1 cM region, in which marker 02302.286 and abcc2 were ≤2.81 cM, and the GBS marker 697 was an estimated 1.89 cM distant from the causal genetic factor. This positional mapping data showed that an O. nubilalis genome region encoding an abcc2 transporter is in proximity to a single QTL involved in the inheritance of Cry1F resistance, and will assist in the future identification the mutation(s) involved with this phenotype.
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U2 - 10.1016/j.ibmb.2015.06.003
DO - 10.1016/j.ibmb.2015.06.003
M3 - Article
C2 - 26093031
AN - SCOPUS:84949304583
SN - 0965-1748
VL - 63
SP - 86
EP - 96
JO - Insect Biochemistry and Molecular Biology
JF - Insect Biochemistry and Molecular Biology
ER -