Linkage relationships and haplotype variation of the major histocompatibility complex class I A genes in the cichlid fish Oreochromis niloticus

Brent W. Murray, Philomeen Nilsson, Zofia Zaleska-Rutczynska, Holger Sültmann, Jan Klein

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The haplochromine cichlid species flocks of the East African Great Lakes are one of the best examples of adaptive radiation. Analysis of genetic variation among these species provides valuable information on species relationships and timing of speciation events. Although the haplochromine cichlids generally display little genetic variation, the major histocompatibility complex (Mhc) genes have been found to be highly variable. A study of the linkage relationships of the Mhc class I A genes in the cichlid fish Oreochromis niloticus was therefore undertaken. Class I loci were identified, and their segregation in seven mothers and their haploid embryos was determined. In total, 56 class I A sequences were found among the seven families. A strong concordance of segregation was observed in five haplotypes among the embryos, indicating a close linkage of all loci. The number of loci per haplotype varied from 11 to 17, while the total number of distinct loci found among all families was 22. These findings show that all class I A loci are linked in a single genetic cluster in O. niloticus.

Original languageEnglish (US)
Pages (from-to)437-448
Number of pages12
JournalMarine Biotechnology
Volume2
Issue number5
DOIs
StatePublished - Jan 1 2000

Fingerprint

vif Genes
Cichlids
major histocompatibility complex
cichlid
Oreochromis niloticus
Major Histocompatibility Complex
Haplotypes
linkage (genetics)
haplotypes
loci
genetic variation
gene
embryo
fish
species flock
adaptive radiation
genes
embryo (animal)
Embryonic Structures
Haploidy

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Aquatic Science

Cite this

Murray, Brent W. ; Nilsson, Philomeen ; Zaleska-Rutczynska, Zofia ; Sültmann, Holger ; Klein, Jan. / Linkage relationships and haplotype variation of the major histocompatibility complex class I A genes in the cichlid fish Oreochromis niloticus. In: Marine Biotechnology. 2000 ; Vol. 2, No. 5. pp. 437-448.
@article{af721645176543a6a5eb9541686563d3,
title = "Linkage relationships and haplotype variation of the major histocompatibility complex class I A genes in the cichlid fish Oreochromis niloticus",
abstract = "The haplochromine cichlid species flocks of the East African Great Lakes are one of the best examples of adaptive radiation. Analysis of genetic variation among these species provides valuable information on species relationships and timing of speciation events. Although the haplochromine cichlids generally display little genetic variation, the major histocompatibility complex (Mhc) genes have been found to be highly variable. A study of the linkage relationships of the Mhc class I A genes in the cichlid fish Oreochromis niloticus was therefore undertaken. Class I loci were identified, and their segregation in seven mothers and their haploid embryos was determined. In total, 56 class I A sequences were found among the seven families. A strong concordance of segregation was observed in five haplotypes among the embryos, indicating a close linkage of all loci. The number of loci per haplotype varied from 11 to 17, while the total number of distinct loci found among all families was 22. These findings show that all class I A loci are linked in a single genetic cluster in O. niloticus.",
author = "Murray, {Brent W.} and Philomeen Nilsson and Zofia Zaleska-Rutczynska and Holger S{\"u}ltmann and Jan Klein",
year = "2000",
month = "1",
day = "1",
doi = "10.1007/s101260000000",
language = "English (US)",
volume = "2",
pages = "437--448",
journal = "Marine Biotechnology",
issn = "1436-2228",
publisher = "Springer New York",
number = "5",

}

Linkage relationships and haplotype variation of the major histocompatibility complex class I A genes in the cichlid fish Oreochromis niloticus. / Murray, Brent W.; Nilsson, Philomeen; Zaleska-Rutczynska, Zofia; Sültmann, Holger; Klein, Jan.

In: Marine Biotechnology, Vol. 2, No. 5, 01.01.2000, p. 437-448.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Linkage relationships and haplotype variation of the major histocompatibility complex class I A genes in the cichlid fish Oreochromis niloticus

AU - Murray, Brent W.

AU - Nilsson, Philomeen

AU - Zaleska-Rutczynska, Zofia

AU - Sültmann, Holger

AU - Klein, Jan

PY - 2000/1/1

Y1 - 2000/1/1

N2 - The haplochromine cichlid species flocks of the East African Great Lakes are one of the best examples of adaptive radiation. Analysis of genetic variation among these species provides valuable information on species relationships and timing of speciation events. Although the haplochromine cichlids generally display little genetic variation, the major histocompatibility complex (Mhc) genes have been found to be highly variable. A study of the linkage relationships of the Mhc class I A genes in the cichlid fish Oreochromis niloticus was therefore undertaken. Class I loci were identified, and their segregation in seven mothers and their haploid embryos was determined. In total, 56 class I A sequences were found among the seven families. A strong concordance of segregation was observed in five haplotypes among the embryos, indicating a close linkage of all loci. The number of loci per haplotype varied from 11 to 17, while the total number of distinct loci found among all families was 22. These findings show that all class I A loci are linked in a single genetic cluster in O. niloticus.

AB - The haplochromine cichlid species flocks of the East African Great Lakes are one of the best examples of adaptive radiation. Analysis of genetic variation among these species provides valuable information on species relationships and timing of speciation events. Although the haplochromine cichlids generally display little genetic variation, the major histocompatibility complex (Mhc) genes have been found to be highly variable. A study of the linkage relationships of the Mhc class I A genes in the cichlid fish Oreochromis niloticus was therefore undertaken. Class I loci were identified, and their segregation in seven mothers and their haploid embryos was determined. In total, 56 class I A sequences were found among the seven families. A strong concordance of segregation was observed in five haplotypes among the embryos, indicating a close linkage of all loci. The number of loci per haplotype varied from 11 to 17, while the total number of distinct loci found among all families was 22. These findings show that all class I A loci are linked in a single genetic cluster in O. niloticus.

UR - http://www.scopus.com/inward/record.url?scp=0033670647&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033670647&partnerID=8YFLogxK

U2 - 10.1007/s101260000000

DO - 10.1007/s101260000000

M3 - Article

AN - SCOPUS:0033670647

VL - 2

SP - 437

EP - 448

JO - Marine Biotechnology

JF - Marine Biotechnology

SN - 1436-2228

IS - 5

ER -