Linking toluene degradation with specific microbial populations in soil

Jessica R. Hanson, Jennifer Macalady, David Harris, Kate M. Scow

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

Phospholipid fatty acid (PLFA) analysis of a soil microbial community was coupled with 13C isotope tracer analysis to measure the community's response to addition of 35 μg of [13C]toluene ml of soil solution-1. After 119 h of incubation with toluene, 96% of the incorporated 13C was detected in only 16 of the total 59 PLFAs (27%) extracted from the soil. Of the total 13C-enriched PLFAs, 85% were identical to the PLFAs contained in a toluene-metabolizing bacterium isolated from the same soil. In contrast, the majority of the soil PLFAs (91%) became labeled when the same soil was incubated with [13C]glucose. Our study showed that coupling 13C tracer analysis with PLFA analysis is an effective technique for distinguishing a specific microbial population involved in metabolism of a labeled substrate in complex environments such as soil.

Original languageEnglish (US)
Pages (from-to)5403-5408
Number of pages6
JournalApplied and environmental microbiology
Volume65
Issue number12
StatePublished - Dec 1 1999

Fingerprint

toluene
Toluene
Soil
degradation
Population
soil
phospholipid
isotope labeling
Phospholipids
Fatty Acids
fatty acid
soil analysis
tracer
soil solution
microbial communities
tracer techniques
community response
Isotopes
microbial community
glucose

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

Hanson, Jessica R. ; Macalady, Jennifer ; Harris, David ; Scow, Kate M. / Linking toluene degradation with specific microbial populations in soil. In: Applied and environmental microbiology. 1999 ; Vol. 65, No. 12. pp. 5403-5408.
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Linking toluene degradation with specific microbial populations in soil. / Hanson, Jessica R.; Macalady, Jennifer; Harris, David; Scow, Kate M.

In: Applied and environmental microbiology, Vol. 65, No. 12, 01.12.1999, p. 5403-5408.

Research output: Contribution to journalArticle

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AU - Harris, David

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