Lipid rafts

Inhibitors of endocytosis of botulinum neurotoxin

Kimberly A. Petro, Cara-Lynne Schengrund

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Methyl-β-cyclodextrin (MβCD) was used to extract cholesterol from plasma membranes of N2a murine neuroblastoma cells. MβCD-disruption of lipid rafts enhanced cleavage of SNAP-25 by BoNT/A, axonogenesis, expression of gangliosides GM1 and GD1a, association of GM1 with the nucleus, and exo- and endo-cytosis. Analysis of endocytosis of an anti-synaptotagmin I monoclonal antibody that recognised part of the protein found on the luminal surface of synaptic vesicles indicated that it was taken up more readily by MβCD-treated cells, supporting the hypothesis that enhanced exocytosis of synaptic vesicles provided more SV2 on the cell surface thereby making more available for toxin binding.

Original languageEnglish (US)
Pages (from-to)358-374
Number of pages17
JournalBotulinum Journal
Volume1
Issue number4
DOIs
StatePublished - Dec 1 2010

Fingerprint

Neurotoxins
Cyclodextrins
Endocytosis
Synaptic Vesicles
Lipids
Synaptotagmin I
G(M1) Ganglioside
Exocytosis
Cell membranes
Neuroblastoma
Cholesterol
Monoclonal Antibodies
Cells
Cell Membrane
Proteins

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cellular and Molecular Neuroscience
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Petro, Kimberly A. ; Schengrund, Cara-Lynne. / Lipid rafts : Inhibitors of endocytosis of botulinum neurotoxin. In: Botulinum Journal. 2010 ; Vol. 1, No. 4. pp. 358-374.
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Lipid rafts : Inhibitors of endocytosis of botulinum neurotoxin. / Petro, Kimberly A.; Schengrund, Cara-Lynne.

In: Botulinum Journal, Vol. 1, No. 4, 01.12.2010, p. 358-374.

Research output: Contribution to journalArticle

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