Lipid rafts: Inhibitors of endocytosis of botulinum neurotoxin

Kimberly A. Petro, Cara Lynne Schengrund

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Methyl-β-cyclodextrin (MβCD) was used to extract cholesterol from plasma membranes of N2a murine neuroblastoma cells. MβCD-disruption of lipid rafts enhanced cleavage of SNAP-25 by BoNT/A, axonogenesis, expression of gangliosides GM1 and GD1a, association of GM1 with the nucleus, and exo- and endo-cytosis. Analysis of endocytosis of an anti-synaptotagmin I monoclonal antibody that recognised part of the protein found on the luminal surface of synaptic vesicles indicated that it was taken up more readily by MβCD-treated cells, supporting the hypothesis that enhanced exocytosis of synaptic vesicles provided more SV2 on the cell surface thereby making more available for toxin binding.

Original languageEnglish (US)
Pages (from-to)358-374
Number of pages17
JournalBotulinum Journal
Volume1
Issue number4
DOIs
StatePublished - 2010

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Cellular and Molecular Neuroscience

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