Liquid-Cell Electron Tomography of Biological Systems

William Dearnaley, Beatrice Schleupner, Cameron Varano, Nick A. Alden, Floricel Gonzalez, Michael A. Casasanta, Birgit E. Scharf, Madeline J. Dukes, Deb Kelly

Research output: Contribution to journalArticle

Abstract

Liquid-cell electron microscopy is a rapidly growing field in the imaging domain. While real-time observations are readily available to analyze materials and biological systems, these measurementshave been limited to the two-dimensional (2-D) image plane. Here, we introduce an exciting technical advance to image materials in 3-D while enclosed in liquid. The development of liquid-cell electron tomography permitted us to observe and quantify host-pathogen interactions in solution while contained in the vacuum system of the electron microscope. In doing so, we demonstrate new insights for the rules of engagement involving a unique bacteriophage and its host bacterium. A deeper analysis of the genetic content of the phage pathogens revealed structural features of the infectious units while introducing a new paradigm for host interactions. Overall, we demonstrate a technological opportunity to elevate research efforts for in situ imaging while providing a new level of dimensionality beyond the current state of the field.

Original languageEnglish (US)
Pages (from-to)6734-6741
Number of pages8
JournalNano letters
Volume19
Issue number10
DOIs
StatePublished - Oct 9 2019

Fingerprint

Biological systems
Tomography
Bacteriophages
pathogens
tomography
Pathogens
Electrons
Liquids
liquids
cells
bacteriophages
Imaging techniques
electrons
vacuum systems
bacteria
Electron microscopy
electron microscopy
Bacteria
Electron microscopes
electron microscopes

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering

Cite this

Dearnaley, William ; Schleupner, Beatrice ; Varano, Cameron ; Alden, Nick A. ; Gonzalez, Floricel ; Casasanta, Michael A. ; Scharf, Birgit E. ; Dukes, Madeline J. ; Kelly, Deb. / Liquid-Cell Electron Tomography of Biological Systems. In: Nano letters. 2019 ; Vol. 19, No. 10. pp. 6734-6741.
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Dearnaley, W, Schleupner, B, Varano, C, Alden, NA, Gonzalez, F, Casasanta, MA, Scharf, BE, Dukes, MJ & Kelly, D 2019, 'Liquid-Cell Electron Tomography of Biological Systems', Nano letters, vol. 19, no. 10, pp. 6734-6741. https://doi.org/10.1021/acs.nanolett.9b01309

Liquid-Cell Electron Tomography of Biological Systems. / Dearnaley, William; Schleupner, Beatrice; Varano, Cameron; Alden, Nick A.; Gonzalez, Floricel; Casasanta, Michael A.; Scharf, Birgit E.; Dukes, Madeline J.; Kelly, Deb.

In: Nano letters, Vol. 19, No. 10, 09.10.2019, p. 6734-6741.

Research output: Contribution to journalArticle

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Dearnaley W, Schleupner B, Varano C, Alden NA, Gonzalez F, Casasanta MA et al. Liquid-Cell Electron Tomography of Biological Systems. Nano letters. 2019 Oct 9;19(10):6734-6741. https://doi.org/10.1021/acs.nanolett.9b01309