A simple and practical method for the cryopreservation of red cells for transfusion has been described. Human red cells suspended in 14% (v/v) glycerol were placed in flat stainless steel containers and frozen by immersion in liquid nitrogen. The frozen blood was stored at -196°C. After thawing, the blood was transferred into plastic blood bags and washed three times, once with mannitol and twice with saline, to remove glycerol and free hemoglobin. Over-all recovery of the cells was greater than 90% and post-transfusion survival of the recovered red cells was comparable to survival of fresh, unfrozen blood. More than 350 units of red cells frozen and thawed by this process have been administered to patients.
All Science Journal Classification (ASJC) codes
- Biochemistry, Genetics and Molecular Biology(all)
- Agricultural and Biological Sciences(all)