Liquid nitrogen preservation of red blood cells for transfusion. A low glycerol - Rapid freeze procedure

Arthur W. Rowe, M. Elaine Eyster, A. Kellner

    Research output: Contribution to journalArticle

    119 Citations (Scopus)

    Abstract

    A simple and practical method for the cryopreservation of red cells for transfusion has been described. Human red cells suspended in 14% (v/v) glycerol were placed in flat stainless steel containers and frozen by immersion in liquid nitrogen. The frozen blood was stored at -196°C. After thawing, the blood was transferred into plastic blood bags and washed three times, once with mannitol and twice with saline, to remove glycerol and free hemoglobin. Over-all recovery of the cells was greater than 90% and post-transfusion survival of the recovered red cells was comparable to survival of fresh, unfrozen blood. More than 350 units of red cells frozen and thawed by this process have been administered to patients.

    Original languageEnglish (US)
    Pages (from-to)119-128
    Number of pages10
    JournalCryobiology
    Volume5
    Issue number2
    DOIs
    StatePublished - Jan 1 1968

    Fingerprint

    Erythrocyte Transfusion
    Liquid nitrogen
    Glycerol
    glycerol
    Blood
    erythrocytes
    Nitrogen
    Cells
    liquids
    nitrogen
    blood
    Steel containers
    cells
    Thawing
    Stainless Steel
    Mannitol
    Survival
    Cryopreservation
    stainless steel
    Immersion

    All Science Journal Classification (ASJC) codes

    • Biochemistry, Genetics and Molecular Biology(all)
    • Agricultural and Biological Sciences(all)

    Cite this

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    abstract = "A simple and practical method for the cryopreservation of red cells for transfusion has been described. Human red cells suspended in 14{\%} (v/v) glycerol were placed in flat stainless steel containers and frozen by immersion in liquid nitrogen. The frozen blood was stored at -196°C. After thawing, the blood was transferred into plastic blood bags and washed three times, once with mannitol and twice with saline, to remove glycerol and free hemoglobin. Over-all recovery of the cells was greater than 90{\%} and post-transfusion survival of the recovered red cells was comparable to survival of fresh, unfrozen blood. More than 350 units of red cells frozen and thawed by this process have been administered to patients.",
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    Liquid nitrogen preservation of red blood cells for transfusion. A low glycerol - Rapid freeze procedure. / Rowe, Arthur W.; Eyster, M. Elaine; Kellner, A.

    In: Cryobiology, Vol. 5, No. 2, 01.01.1968, p. 119-128.

    Research output: Contribution to journalArticle

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    AU - Eyster, M. Elaine

    AU - Kellner, A.

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