Listeria monocytogenes in stone fruits linked to a multistate outbreak: Enumeration of cells and whole-genome sequencing

Yi Chen, Laurel S. Burall, Yan Luo, Ruth Timme, David Melka, Tim Muruvanda, Justin Payne, Charles Wang, George Kastanis, Anna Maounounen-Laasri, Antonio J. De Jesus, Phillip E. Curry, Robert Stones, Okumu K'Aluoch, Eileen Liu, Monique Salter, Thomas S. Hammack, Peter S. Evans, Mickey Parish, Marc W. AllardAtin Datta, Errol A. Strain, Eric W. Brown

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Abstract

In 2014, the identification of stone fruits contaminated with Listeria monocytogenes led to the subsequent identification of a multistate outbreak. Simultaneous detection and enumeration of L. monocytogenes were performed on 105 fruits, each weighing 127 to 145 g, collected from 7 contaminated lots. The results showed that 53.3% of the fruits yielded L. monocytogenes (lower limit of detection, 5 CFU/fruit), and the levels ranged from 5 to 2,850 CFU/fruit, with a geometric mean of 11.3 CFU/fruit (0.1 CFU/g of fruit). Two serotypes, IVb-v1 and 1/2b, were identified by a combination of PCR- and antiserum-based serotyping among isolates from fruits and their packing environment; certain fruits contained a mixture of both serotypes. Single nucleotide polymorphism (SNP)-based whole-genome sequencing (WGS) analysis clustered isolates from two case-patients with the serotype IVb-v1 isolates and distinguished outbreak-associated isolates from pulsed-field gel electrophoresis (PFGE)-matched, but epidemiologically unrelated, clinical isolates. The outbreak-associated isolates differed by up to 42 SNPs. All but one serotype 1/2b isolate formed another WGS cluster and differed by up to 17 SNPs. Fully closed genomes of isolates from the stone fruits were used as references to maximize the resolution and to increase our confidence in prophage analysis. Putative prophages were conserved among isolates of each WGS cluster. All serotype IVb-v1 isolates belonged to singleton sequence type 382 (ST382); all but one serotype 1/2b isolate belonged to clonal complex 5.

Original languageEnglish (US)
Pages (from-to)7030-7040
Number of pages11
JournalApplied and environmental microbiology
Volume82
Issue number24
DOIs
StatePublished - 2016

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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    Chen, Y., Burall, L. S., Luo, Y., Timme, R., Melka, D., Muruvanda, T., Payne, J., Wang, C., Kastanis, G., Maounounen-Laasri, A., De Jesus, A. J., Curry, P. E., Stones, R., K'Aluoch, O., Liu, E., Salter, M., Hammack, T. S., Evans, P. S., Parish, M., ... Brown, E. W. (2016). Listeria monocytogenes in stone fruits linked to a multistate outbreak: Enumeration of cells and whole-genome sequencing. Applied and environmental microbiology, 82(24), 7030-7040. https://doi.org/10.1128/AEM.01486-16