Lithographically defined two- and three-dimensional tissue microarrays.

Esther Winter Gomez, Celeste M. Nelson

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Traditional methods to study normal and pathological development of tissues have been limited by -difficulties in controlling experimental conditions and quantifying biological processes of interest. Here we describe methods to create microarrays of engineered tissues that enable controlled and quantitative investigations. Using soft lithography-based techniques, extracellular matrix proteins can be microcontact printed or micromolded to make two- and three-dimensional micropatterned scaffolds. The ultimate form and resulting properties of the tissue construct are dictated by the geometry of the patterned extracellular matrix components. This chapter describes elastomeric stamp fabrication, microcontact printing and micromolding of extracellular matrix proteins, cell culture in micropatterned substrata, and quantitative immunofluorescence analysis of micropatterned tissues.

Original languageEnglish (US)
Pages (from-to)107-116
Number of pages10
JournalMethods in molecular biology (Clifton, N.J.)
Volume671
StatePublished - Jan 1 2011

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Extracellular Matrix Proteins
Biological Phenomena
Printing
Fluorescent Antibody Technique
Extracellular Matrix
Cell Culture Techniques
elastomeric

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

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Lithographically defined two- and three-dimensional tissue microarrays. / Gomez, Esther Winter; Nelson, Celeste M.

In: Methods in molecular biology (Clifton, N.J.), Vol. 671, 01.01.2011, p. 107-116.

Research output: Contribution to journalArticle

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