Lithographically patterned channels spatially segregate kinesin motor activity and effectively guide microtubule movements

Samira G. Moorjani, Lili Jia, Thomas N. Jackson, William O. Hancock

Research output: Contribution to journalArticlepeer-review

112 Scopus citations

Abstract

To extract useful work from biological motor proteins, it is necessary to orient microtubules traveling over kinesin-coated surfaces properly. Toward this goal, we have used microfabrication to construct 1.5-μm-deep channels in SU-8 photoresist patterned on glass. Although motor proteins bind to all surfaces, these channels localize motility exclusively to the glass surface, and the photoresist creates steep walls that direct microtubule movement. This technique provides a general approach for lithographically patterning enzyme activity.

Original languageEnglish (US)
Pages (from-to)633-637
Number of pages5
JournalNano letters
Volume3
Issue number5
DOIs
StatePublished - May 1 2003

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering

Fingerprint Dive into the research topics of 'Lithographically patterned channels spatially segregate kinesin motor activity and effectively guide microtubule movements'. Together they form a unique fingerprint.

Cite this