Local auxin biosynthesis mediated by a yucca flavin monooxygenase regulates haustorium development in the parasitic plant phtheirospermum japonicum

Juliane K. Ishida, Takanori Wakatake, Satoko Yoshida, Yumiko Takebayashi, Hiroyuki Kasahara, Eric Wafula, Claude W. Depamphilis, Shigetou Namba, Ken Shirasu

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Parasitic plants in the Orobanchaceae cause serious agricultural problems worldwide. Parasitic plants develop a multicellular infectious organ called a haustorium after recognition of host-released signals. To understand the molecular events associated with host signal perception and haustorium development, we identified differentially regulated genes expressed during early haustorium development in the facultative parasite Phtheirospermum japonicum using a de novo assembled transcriptome and a customized microarray. Among the genes that were upregulated during early haustorium development, we identified YUC3, which encodes a functional YUCCA (YUC) flavin monooxygenase involved in auxin biosynthesis. YUC3 was specifically expressed in the epidermal cells around the host contact site at an early time point in haustorium formation. The spatio-temporal expression patterns of YUC3 coincided with those of the auxin response marker DR5, suggesting generation of auxin response maxima at the haustorium apex. Roots transformed with YUC3 knockdown constructs formed haustoria less frequently than nontransgenic roots. Moreover, ectopic expression of YUC3 at the root epidermal cells induced the formation of haustorium-like structures in transgenic P. japonicum roots. Our results suggest that expression of the auxin biosynthesis gene YUC3 at the epidermal cells near the contact site plays a pivotal role in haustorium formation in the root parasitic plant P. japonicum.

Original languageEnglish (US)
Pages (from-to)1795-1814
Number of pages20
JournalPlant Cell
Volume28
Issue number8
DOIs
StatePublished - Aug 2016

Fingerprint

Yucca
flavins
Indoleacetic Acids
parasitic plants
Mixed Function Oxygenases
auxins
biosynthesis
Orobanchaceae
early development
Genes
Plant Roots
genes
cells
Transcriptome
transcriptome
Parasites
genetically modified organisms
parasites
4,6-dinitro-o-cresol

All Science Journal Classification (ASJC) codes

  • Plant Science
  • Cell Biology

Cite this

Ishida, J. K., Wakatake, T., Yoshida, S., Takebayashi, Y., Kasahara, H., Wafula, E., ... Shirasu, K. (2016). Local auxin biosynthesis mediated by a yucca flavin monooxygenase regulates haustorium development in the parasitic plant phtheirospermum japonicum. Plant Cell, 28(8), 1795-1814. https://doi.org/10.1105/tpc.16.00310
Ishida, Juliane K. ; Wakatake, Takanori ; Yoshida, Satoko ; Takebayashi, Yumiko ; Kasahara, Hiroyuki ; Wafula, Eric ; Depamphilis, Claude W. ; Namba, Shigetou ; Shirasu, Ken. / Local auxin biosynthesis mediated by a yucca flavin monooxygenase regulates haustorium development in the parasitic plant phtheirospermum japonicum. In: Plant Cell. 2016 ; Vol. 28, No. 8. pp. 1795-1814.
@article{f91c03b10b4d4e139305c08dc26a9235,
title = "Local auxin biosynthesis mediated by a yucca flavin monooxygenase regulates haustorium development in the parasitic plant phtheirospermum japonicum",
abstract = "Parasitic plants in the Orobanchaceae cause serious agricultural problems worldwide. Parasitic plants develop a multicellular infectious organ called a haustorium after recognition of host-released signals. To understand the molecular events associated with host signal perception and haustorium development, we identified differentially regulated genes expressed during early haustorium development in the facultative parasite Phtheirospermum japonicum using a de novo assembled transcriptome and a customized microarray. Among the genes that were upregulated during early haustorium development, we identified YUC3, which encodes a functional YUCCA (YUC) flavin monooxygenase involved in auxin biosynthesis. YUC3 was specifically expressed in the epidermal cells around the host contact site at an early time point in haustorium formation. The spatio-temporal expression patterns of YUC3 coincided with those of the auxin response marker DR5, suggesting generation of auxin response maxima at the haustorium apex. Roots transformed with YUC3 knockdown constructs formed haustoria less frequently than nontransgenic roots. Moreover, ectopic expression of YUC3 at the root epidermal cells induced the formation of haustorium-like structures in transgenic P. japonicum roots. Our results suggest that expression of the auxin biosynthesis gene YUC3 at the epidermal cells near the contact site plays a pivotal role in haustorium formation in the root parasitic plant P. japonicum.",
author = "Ishida, {Juliane K.} and Takanori Wakatake and Satoko Yoshida and Yumiko Takebayashi and Hiroyuki Kasahara and Eric Wafula and Depamphilis, {Claude W.} and Shigetou Namba and Ken Shirasu",
year = "2016",
month = "8",
doi = "10.1105/tpc.16.00310",
language = "English (US)",
volume = "28",
pages = "1795--1814",
journal = "Plant Cell",
issn = "1040-4651",
publisher = "American Society of Plant Biologists",
number = "8",

}

Ishida, JK, Wakatake, T, Yoshida, S, Takebayashi, Y, Kasahara, H, Wafula, E, Depamphilis, CW, Namba, S & Shirasu, K 2016, 'Local auxin biosynthesis mediated by a yucca flavin monooxygenase regulates haustorium development in the parasitic plant phtheirospermum japonicum', Plant Cell, vol. 28, no. 8, pp. 1795-1814. https://doi.org/10.1105/tpc.16.00310

Local auxin biosynthesis mediated by a yucca flavin monooxygenase regulates haustorium development in the parasitic plant phtheirospermum japonicum. / Ishida, Juliane K.; Wakatake, Takanori; Yoshida, Satoko; Takebayashi, Yumiko; Kasahara, Hiroyuki; Wafula, Eric; Depamphilis, Claude W.; Namba, Shigetou; Shirasu, Ken.

In: Plant Cell, Vol. 28, No. 8, 08.2016, p. 1795-1814.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Local auxin biosynthesis mediated by a yucca flavin monooxygenase regulates haustorium development in the parasitic plant phtheirospermum japonicum

AU - Ishida, Juliane K.

AU - Wakatake, Takanori

AU - Yoshida, Satoko

AU - Takebayashi, Yumiko

AU - Kasahara, Hiroyuki

AU - Wafula, Eric

AU - Depamphilis, Claude W.

AU - Namba, Shigetou

AU - Shirasu, Ken

PY - 2016/8

Y1 - 2016/8

N2 - Parasitic plants in the Orobanchaceae cause serious agricultural problems worldwide. Parasitic plants develop a multicellular infectious organ called a haustorium after recognition of host-released signals. To understand the molecular events associated with host signal perception and haustorium development, we identified differentially regulated genes expressed during early haustorium development in the facultative parasite Phtheirospermum japonicum using a de novo assembled transcriptome and a customized microarray. Among the genes that were upregulated during early haustorium development, we identified YUC3, which encodes a functional YUCCA (YUC) flavin monooxygenase involved in auxin biosynthesis. YUC3 was specifically expressed in the epidermal cells around the host contact site at an early time point in haustorium formation. The spatio-temporal expression patterns of YUC3 coincided with those of the auxin response marker DR5, suggesting generation of auxin response maxima at the haustorium apex. Roots transformed with YUC3 knockdown constructs formed haustoria less frequently than nontransgenic roots. Moreover, ectopic expression of YUC3 at the root epidermal cells induced the formation of haustorium-like structures in transgenic P. japonicum roots. Our results suggest that expression of the auxin biosynthesis gene YUC3 at the epidermal cells near the contact site plays a pivotal role in haustorium formation in the root parasitic plant P. japonicum.

AB - Parasitic plants in the Orobanchaceae cause serious agricultural problems worldwide. Parasitic plants develop a multicellular infectious organ called a haustorium after recognition of host-released signals. To understand the molecular events associated with host signal perception and haustorium development, we identified differentially regulated genes expressed during early haustorium development in the facultative parasite Phtheirospermum japonicum using a de novo assembled transcriptome and a customized microarray. Among the genes that were upregulated during early haustorium development, we identified YUC3, which encodes a functional YUCCA (YUC) flavin monooxygenase involved in auxin biosynthesis. YUC3 was specifically expressed in the epidermal cells around the host contact site at an early time point in haustorium formation. The spatio-temporal expression patterns of YUC3 coincided with those of the auxin response marker DR5, suggesting generation of auxin response maxima at the haustorium apex. Roots transformed with YUC3 knockdown constructs formed haustoria less frequently than nontransgenic roots. Moreover, ectopic expression of YUC3 at the root epidermal cells induced the formation of haustorium-like structures in transgenic P. japonicum roots. Our results suggest that expression of the auxin biosynthesis gene YUC3 at the epidermal cells near the contact site plays a pivotal role in haustorium formation in the root parasitic plant P. japonicum.

UR - http://www.scopus.com/inward/record.url?scp=84984788508&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84984788508&partnerID=8YFLogxK

U2 - 10.1105/tpc.16.00310

DO - 10.1105/tpc.16.00310

M3 - Article

C2 - 27385817

AN - SCOPUS:84984788508

VL - 28

SP - 1795

EP - 1814

JO - Plant Cell

JF - Plant Cell

SN - 1040-4651

IS - 8

ER -