Localization of common cytotoxic T lymphocyte recognition epitopes on Simian papovavirus sv40 and human papovavirus JC virus T antigens

Alison M. Deckhut, Mary J. Tevethia, Sheryl Haggerty, Richard J. Frisque, Satvir Stevethia

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Human papovavirus 1C virus (JCV) and Simian virus 40 (SV40) tumor or T antigens demonstrate considerable sequence homology which is reflected by antibody cross-reactivity. This similarity raised the possibility that JCV and SV40 T antigen also might contain common cytotoxic T lymphocyte (CTL) recognition epitopes. In this study we identified and mapped such sites on the JCV T antigen. C57BI/6 cell lines transformed by JCV/SV40 T antigen chimeras were generated and tested for susceptibility to lysis by five H-2b restricted SV40-specific CTL clones: Y-1, Y-2, Y-3, Y-4, and Y-5. These CTL clones recognize specific epitopes within amino acids 205-219 (site I), 220-233 (sites 11 and III), 369-511 (site IV), and 489-503 (site V) on SV40 T Ag, respectively. The results show that sites I, 11, III, and IV (recognized by CTL clones Y-1, Y-2, Y-3, and Y-4, respectively) represent common epitopes on SV40 and JCV T antigens. CTL clone Y-5 failed to recognize JCV T antigen indicating that CTL can discriminate between the two antigenically related T antigens.

Original languageEnglish (US)
Pages (from-to)122-132
Number of pages11
JournalVirology
Volume183
Issue number1
DOIs
StatePublished - Jul 1991

All Science Journal Classification (ASJC) codes

  • Virology

Fingerprint Dive into the research topics of 'Localization of common cytotoxic T lymphocyte recognition epitopes on Simian papovavirus sv40 and human papovavirus JC virus T antigens'. Together they form a unique fingerprint.

Cite this