Localization of eukaryote-specific ribosomal proteins in a 5.5-Å cryo-EM map of the 80S eukaryotic ribosome

Jean Paul Armache, Alexander Jarasch, Andreas M. Anger, Elizabeth Villa, Thomas Becker, Shashi Bhushan, Fabrice Jossinet, Michael Habeck, Gülcin Dindar, Sibylle Franckenberg, Viter Marquez, Thorsten Mielke, Michael Thomm, Otto Berninghausen, Birgitta Beatrix, Johannes Söding, Eric Westhof, Daniel N. Wilson, Roland Beckmann

Research output: Contribution to journalArticle

94 Citations (Scopus)

Abstract

Protein synthesis in all living organisms occurs on ribonucleoprotein particles, called ribosomes. Despite the universality of this process, eukaryotic ribosomes are significantly larger in size than their bacterial counterparts due in part to the presence of 80 r proteins rather than 54 in bacteria. Using cryoelectron microscopy reconstructions of a translating plant (Triticum aestivum) 80S ribosome at 5.5-Å resolution, together with a 6.1-Å map of a translating Saccharomyces cerevisiae 80S ribosome, we have localized and modeled 74/80 (92.5%) of the ribosomal proteins, encompassing 12 archaeal/eukaryote-specific small subunit proteins as well as the complete complement of the ribosomal proteins of the eukaryotic large subunit. Near-complete atomic models of the 80S ribo- some provide insights into the structure, function, and evolution of the eukaryotic translational apparatus.

Original languageEnglish (US)
Pages (from-to)19754-19759
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume107
Issue number46
DOIs
StatePublished - Nov 16 2010

Fingerprint

Ribosomal Proteins
Eukaryota
Ribosomes
Cryoelectron Microscopy
Ribonucleoproteins
Protein Subunits
Triticum
Saccharomyces cerevisiae
Complement System Proteins
Proteins
Bacteria

All Science Journal Classification (ASJC) codes

  • General

Cite this

Armache, Jean Paul ; Jarasch, Alexander ; Anger, Andreas M. ; Villa, Elizabeth ; Becker, Thomas ; Bhushan, Shashi ; Jossinet, Fabrice ; Habeck, Michael ; Dindar, Gülcin ; Franckenberg, Sibylle ; Marquez, Viter ; Mielke, Thorsten ; Thomm, Michael ; Berninghausen, Otto ; Beatrix, Birgitta ; Söding, Johannes ; Westhof, Eric ; Wilson, Daniel N. ; Beckmann, Roland. / Localization of eukaryote-specific ribosomal proteins in a 5.5-Å cryo-EM map of the 80S eukaryotic ribosome. In: Proceedings of the National Academy of Sciences of the United States of America. 2010 ; Vol. 107, No. 46. pp. 19754-19759.
@article{5618a1cec84e4179b4f4e966f3ba3384,
title = "Localization of eukaryote-specific ribosomal proteins in a 5.5-{\AA} cryo-EM map of the 80S eukaryotic ribosome",
abstract = "Protein synthesis in all living organisms occurs on ribonucleoprotein particles, called ribosomes. Despite the universality of this process, eukaryotic ribosomes are significantly larger in size than their bacterial counterparts due in part to the presence of 80 r proteins rather than 54 in bacteria. Using cryoelectron microscopy reconstructions of a translating plant (Triticum aestivum) 80S ribosome at 5.5-{\AA} resolution, together with a 6.1-{\AA} map of a translating Saccharomyces cerevisiae 80S ribosome, we have localized and modeled 74/80 (92.5{\%}) of the ribosomal proteins, encompassing 12 archaeal/eukaryote-specific small subunit proteins as well as the complete complement of the ribosomal proteins of the eukaryotic large subunit. Near-complete atomic models of the 80S ribo- some provide insights into the structure, function, and evolution of the eukaryotic translational apparatus.",
author = "Armache, {Jean Paul} and Alexander Jarasch and Anger, {Andreas M.} and Elizabeth Villa and Thomas Becker and Shashi Bhushan and Fabrice Jossinet and Michael Habeck and G{\"u}lcin Dindar and Sibylle Franckenberg and Viter Marquez and Thorsten Mielke and Michael Thomm and Otto Berninghausen and Birgitta Beatrix and Johannes S{\"o}ding and Eric Westhof and Wilson, {Daniel N.} and Roland Beckmann",
year = "2010",
month = "11",
day = "16",
doi = "10.1073/pnas.1010005107",
language = "English (US)",
volume = "107",
pages = "19754--19759",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "46",

}

Armache, JP, Jarasch, A, Anger, AM, Villa, E, Becker, T, Bhushan, S, Jossinet, F, Habeck, M, Dindar, G, Franckenberg, S, Marquez, V, Mielke, T, Thomm, M, Berninghausen, O, Beatrix, B, Söding, J, Westhof, E, Wilson, DN & Beckmann, R 2010, 'Localization of eukaryote-specific ribosomal proteins in a 5.5-Å cryo-EM map of the 80S eukaryotic ribosome', Proceedings of the National Academy of Sciences of the United States of America, vol. 107, no. 46, pp. 19754-19759. https://doi.org/10.1073/pnas.1010005107

Localization of eukaryote-specific ribosomal proteins in a 5.5-Å cryo-EM map of the 80S eukaryotic ribosome. / Armache, Jean Paul; Jarasch, Alexander; Anger, Andreas M.; Villa, Elizabeth; Becker, Thomas; Bhushan, Shashi; Jossinet, Fabrice; Habeck, Michael; Dindar, Gülcin; Franckenberg, Sibylle; Marquez, Viter; Mielke, Thorsten; Thomm, Michael; Berninghausen, Otto; Beatrix, Birgitta; Söding, Johannes; Westhof, Eric; Wilson, Daniel N.; Beckmann, Roland.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, No. 46, 16.11.2010, p. 19754-19759.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Localization of eukaryote-specific ribosomal proteins in a 5.5-Å cryo-EM map of the 80S eukaryotic ribosome

AU - Armache, Jean Paul

AU - Jarasch, Alexander

AU - Anger, Andreas M.

AU - Villa, Elizabeth

AU - Becker, Thomas

AU - Bhushan, Shashi

AU - Jossinet, Fabrice

AU - Habeck, Michael

AU - Dindar, Gülcin

AU - Franckenberg, Sibylle

AU - Marquez, Viter

AU - Mielke, Thorsten

AU - Thomm, Michael

AU - Berninghausen, Otto

AU - Beatrix, Birgitta

AU - Söding, Johannes

AU - Westhof, Eric

AU - Wilson, Daniel N.

AU - Beckmann, Roland

PY - 2010/11/16

Y1 - 2010/11/16

N2 - Protein synthesis in all living organisms occurs on ribonucleoprotein particles, called ribosomes. Despite the universality of this process, eukaryotic ribosomes are significantly larger in size than their bacterial counterparts due in part to the presence of 80 r proteins rather than 54 in bacteria. Using cryoelectron microscopy reconstructions of a translating plant (Triticum aestivum) 80S ribosome at 5.5-Å resolution, together with a 6.1-Å map of a translating Saccharomyces cerevisiae 80S ribosome, we have localized and modeled 74/80 (92.5%) of the ribosomal proteins, encompassing 12 archaeal/eukaryote-specific small subunit proteins as well as the complete complement of the ribosomal proteins of the eukaryotic large subunit. Near-complete atomic models of the 80S ribo- some provide insights into the structure, function, and evolution of the eukaryotic translational apparatus.

AB - Protein synthesis in all living organisms occurs on ribonucleoprotein particles, called ribosomes. Despite the universality of this process, eukaryotic ribosomes are significantly larger in size than their bacterial counterparts due in part to the presence of 80 r proteins rather than 54 in bacteria. Using cryoelectron microscopy reconstructions of a translating plant (Triticum aestivum) 80S ribosome at 5.5-Å resolution, together with a 6.1-Å map of a translating Saccharomyces cerevisiae 80S ribosome, we have localized and modeled 74/80 (92.5%) of the ribosomal proteins, encompassing 12 archaeal/eukaryote-specific small subunit proteins as well as the complete complement of the ribosomal proteins of the eukaryotic large subunit. Near-complete atomic models of the 80S ribo- some provide insights into the structure, function, and evolution of the eukaryotic translational apparatus.

UR - http://www.scopus.com/inward/record.url?scp=78650532579&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78650532579&partnerID=8YFLogxK

U2 - 10.1073/pnas.1010005107

DO - 10.1073/pnas.1010005107

M3 - Article

C2 - 20974910

AN - SCOPUS:78650532579

VL - 107

SP - 19754

EP - 19759

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 46

ER -