Localization of nucleoside triphosphatase activity to the inner nuclear envelope and associated heterochromatin

Gary Clawson, Daniel S. Friend, Edward A. Smuckler

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Nuclear-envelope nucleoside triphosphatase activity (NTPase), an enzymatic activity thought to participate in RNA transport, was localized in rat liver in situ after brief perfusion with 3% paraformaldehyde. Reaction product was distributed along the nucleoplasmic side of the nuclear envelope (NE) in heterochromatin, was only occasionally found at nuclear pores, and nuclear deposition was selectively blocked by inhibitors of NE NTPase activity. Our results suggest that NTPases, which are active in the NE and which participate in RNA transport, are not specifically associated with nuclear-pore complexes.

Original languageEnglish (US)
Pages (from-to)310-314
Number of pages5
JournalExperimental Cell Research
Volume155
Issue number1
DOIs
StatePublished - Nov 1984

All Science Journal Classification (ASJC) codes

  • Cell Biology

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