Localization of pulmonary surfactant proteins using immunohistochemistry and tissue in situ hybridization

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Abstract

Surfactant, a complex mixture of lipids and proteins, is produced by the type II alveolar epithelial cells. Numerous studies have localized surfactant protein A (SP-A) to type II cells of the lung, and recent studies have shown that the type II cells in the human lung are also the site of synthesis of SP-B, one of the hydrophobic surfactant proteins. There have been conflicting reports about additional sites of SP-A production. We have studied the localization of the mRNAs for SP-A, SP-B, and SP-C in the rat and for SP-C in the human lung by tissue in situ hybridization using cRNA probes. The mRNAs for all three rat surfactant proteins and for human SP-C were found in type II alveolar epithelial cells. In addition, the mRNAs for rat SP-A and SP-B were found in non-ciliated bronchiolar cells. SP-C mRM was not detectable in the bronchiolar cells of both rat and human lung tissue. Immunohistochemical studies in the rat lung with untisera to SP-A and SP-B confirmed the presence of the protein in cell types where the mRNA was found, as well as in some alveolar macrophages. Alveolar macrophages in both rat and human lung tissue were negative for all mRNAs. Further studies are required to ascertain whether there are differences in the processing, function, and regulation of these proteins in the different cell types that produce them.

Original languageEnglish (US)
Pages (from-to)985-995
Number of pages11
JournalExperimental Lung Research
Volume17
Issue number6
DOIs
StatePublished - Jan 1 1991

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Pulmonary Surfactant-Associated Proteins
Pulmonary Surfactant-Associated Protein A
In Situ Hybridization
Rats
Pulmonary Surfactant-Associated Protein B
Immunohistochemistry
Tissue
Lung
Messenger RNA
Surface-Active Agents
Alveolar Epithelial Cells
Alveolar Macrophages
Proteins
Complementary RNA
Complex Mixtures
Lipids
Processing

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Pulmonary and Respiratory Medicine
  • Clinical Biochemistry

Cite this

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abstract = "Surfactant, a complex mixture of lipids and proteins, is produced by the type II alveolar epithelial cells. Numerous studies have localized surfactant protein A (SP-A) to type II cells of the lung, and recent studies have shown that the type II cells in the human lung are also the site of synthesis of SP-B, one of the hydrophobic surfactant proteins. There have been conflicting reports about additional sites of SP-A production. We have studied the localization of the mRNAs for SP-A, SP-B, and SP-C in the rat and for SP-C in the human lung by tissue in situ hybridization using cRNA probes. The mRNAs for all three rat surfactant proteins and for human SP-C were found in type II alveolar epithelial cells. In addition, the mRNAs for rat SP-A and SP-B were found in non-ciliated bronchiolar cells. SP-C mRM was not detectable in the bronchiolar cells of both rat and human lung tissue. Immunohistochemical studies in the rat lung with untisera to SP-A and SP-B confirmed the presence of the protein in cell types where the mRNA was found, as well as in some alveolar macrophages. Alveolar macrophages in both rat and human lung tissue were negative for all mRNAs. Further studies are required to ascertain whether there are differences in the processing, function, and regulation of these proteins in the different cell types that produce them.",
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