Localized Quorum Sensing in Vibrio fischeri

Mary E. Parent, Charles E. Snyder, Nathaniel D. Kopp, Darrell Velegol

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Quorum sensing is almost always regarded as a population density effect in three-dimensional bulk samples of bacteria. Here we create two-dimensional samples of Vibrio fischeri cells adhered onto glass surfaces to examine the effect of local population densities on quorum sensing. This is done by measuring the luminescent response. The 2-D bacterial populations enable us to simultaneously account for time and distance effects on quorum sensing, which were previously very challenging to access in typical three-dimensional bulk samples. Thus, we are able to consider quorum sensing in terms of signal diffusion. A diffusion model of quorum sensing signals guides the experiments and shows that for a given cell spacing (density) and diffusion time there exists a "true quorum"- a number of cells necessary for quorum sensing. We find that quorum sensing can occur locally in 2-D surface samples and is a function of cell population density as well as signal diffusion time.

Original languageEnglish (US)
Pages (from-to)180-187
Number of pages8
JournalColloids and Surfaces B: Biointerfaces
Volume62
Issue number2
DOIs
StatePublished - Apr 1 2008

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

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