Locations of the hydrogenases of Methanobacterium formicicum after subcellular fractionation of cell extract.

S. F. Baron, D. P. Brown, J. G. Ferry

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

The F420 hydrogenase of Methanobacterium formicicum was associated with membranes isolated by sucrose density gradient ultracentrifugation of cell extract. The methyl viologen hydrogenase was present in the soluble fractions. Column chromatography with phenyl-Sepharose CL-4B revealed that the F420 hydrogenase was strongly hydrophobic, suggesting that it associates with isolated membranes through hydrophobic interactions.

Original languageEnglish (US)
Pages (from-to)3823-3825
Number of pages3
JournalJournal of bacteriology
Volume169
Issue number8
DOIs
StatePublished - Aug 1987

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

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